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Title: Zoeken naar het 'Quorum Sensing' system van Cupriavidus metallidurans CH34 en de functie ervan op aarde en in de ruimte
Author(s): Moors, Hugo
De Boever, Patrick
Vanderleyden, Jos
Keywords: Quorum Sensing
Cupriavidus metallidurans CH34
Ralstonia Solanacearum
bacterial behaviour
micro array
Issue Date: Jul-2007
Publication type: Thesis
Citation: Moors H.- Zoeken naar het 'Quorum Sensing' system van Cupriavidus metallidurans CH34 en de functie ervan op aarde en in de ruimte.- Leuven, Belgium: Katholieke Universiteit Leuven, 2007.- 84 p.- Master thesis
Abstract: Sometimes, the behaviour of micro-organisms in space changes in a negative way. They become more virulent. This is of great concern in space research. On earth bacterial virulence is often influenced by a process called 'Quorum Sensing', a bacterial communication system that is controlled by population density. Understanding how QS-systems work might provide important insights on the functioning of changes in bacterial behaviour. In this thesis the QS-system of Cupriavidus metallidurans CH34 is investigated. This is done in silico with bio-informatic tools and experimentally by comparing the wild type CH34 with a PhcA QS-mutant strain of C. metallidurans using laboratory techniques like, micro-array, macroscopic swim tests, bio-assay, growth speed monitoring and carbon source consumption. First of all, the remarkable resemblance of the QS-systems of C. metallidurans and Ralstonia solanacearum is demonstrated. In C. metallidurans two metabolic processes, rubisco and hydrogen oxidation are activated by its QS-system. Also motility is enhanced in the presence of a high PhcA concentration, the QS-control protein. We discovered that the transcription factor VsrD is down-regulated and, consequently, becomes part of the PhcA regulon. The carbon source, gluconate, consumption seems not affected by QS-signalling.
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