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Title: Etude des conditions spatiales sur le développement embryonnaire de la souris
Author(s): Hautefelt, Julien
Baatout, Sarah
Keywords: radiation
developmental biology
Issue Date: 22-Jun-2008
Publication type: Promoted work
Citation: Hautefelt J. - Etude des conditions spatiales sur le développement embryonnaire de la souris - to be implemented.
Abstract: Radiation and microgravity are amongst the most important stresses in space conditions. Gravity is universally present on Earth. It determines the vertical orientation of all living organisms, thus their proper development. The purpose of this study is to decipher the effects of simulated space conditions (for microgravity and irradiation) on the morphology, physiology and gene expression of the mouse embryo and fetus. Two developmental periods have been selected within this study: the preimplantation period (from fertilisation up to the blastula stage) and late organogenesis (with fetal fibroblastic cells harvested at day 19 post conception for performing primary cultures). The STO cell line originating from mouse embryonic fibroblasts is used to optimise and set-up experimental conditions. To simulate microgravity, the desktop Random Positioning Machine (RPM) is being used. The radiation conditions on board of the International Space Station are simulated by applying similar dose rates using a mixture of gamma (Cs-137) and neutron (Cf-252) sources. With the microgravity and radiation facilities available at SCK•CEN, it is therefore possible to mimic an environment close to space conditions. Flow cytometry is then applied to quantify possible space-induced apoptosis, Bcl-2 regulation and caspase-3 activation as well as potential effect on the cell cycle.
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