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Title:	Molecular characterization of the life support bacterium Rhodospirillum rubrum S1H cultivated under space related environmental conditions
Author(s):	Mastroleo, Felice Wattiez, Ruddy Van Houdt, Rob
Keywords:	Rhodospirillum rubrum S1H transcriptomics proteomics space flight ionizing radiation random positioning machine rotating wall vessel MELiSSA
Issue Date:	23-Jan-2009
Publication type:	Thesis
Citation:	Mastroleo F.- Molecular characterization of the life support bacterium Rhodospirillum rubrum S1H cultivated under space related environmental conditions.- Mons, Belgium: University of Mons-Hainaut, 2009.- 171 p.- PhD thesis
Abstract:	R. rubrum S1H inoculated on solid agar medium in dark aerobic conditions was sent to the International space station. Moreover, in an effort to identify a specific response of R. rubrum to space flight, ground simulation of space ionizing radiation and space gravity were performed under identical culture setup and growth conditions encountered during the actual space flight experiment. The cultures were analysed and compared with their corresponding control at both the transcriptomic and proteomic level. In addition, R. rubrum whole protein content was further characterized using a multidimensional protein identification technology (MudPIT). We could put forward the importance of the medium composition and the culture setup on the response of the bacterium to the space flight related stress. We showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. On the other hand, the MudPIT approach permitted us to identify about 25 % (i.e. 1,007 proteins) of R. rubrum's proteome.
Persistent URL:	http://hdl.handle.net/10038/994

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