A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness

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A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness. / Grimm, Daniela; Infanger, Manfred; Westphal, Kris; Ulbrich, Claudia; Pietsch, Jessica; Kossmehl, Peter; Vadrucci, Sonia; Baatout, Sarah; Flick, Burkhard; Paul, Martin; Bauer, Johann; Beck, Michaël (Peer reviewer); Ghardi, Myriam (Peer reviewer).

In: Tissue Engineering Part A, Vol. 15, No. 8, 03.02.2009, p. 2267-2275.

Research output: Contribution to journalArticle

Harvard

Grimm, D, Infanger, M, Westphal, K, Ulbrich, C, Pietsch, J, Kossmehl, P, Vadrucci, S, Baatout, S, Flick, B, Paul, M, Bauer, J, Beck, M & Ghardi, M 2009, 'A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness', Tissue Engineering Part A, vol. 15, no. 8, pp. 2267-2275. https://doi.org/10.1089/ten.tea.2008.0576

APA

Grimm, D., Infanger, M., Westphal, K., Ulbrich, C., Pietsch, J., Kossmehl, P., ... Ghardi, M. (2009). A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness. Tissue Engineering Part A, 15(8), 2267-2275. https://doi.org/10.1089/ten.tea.2008.0576

Vancouver

Grimm D, Infanger M, Westphal K, Ulbrich C, Pietsch J, Kossmehl P et al. A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness. Tissue Engineering Part A. 2009 Feb 3;15(8):2267-2275. https://doi.org/10.1089/ten.tea.2008.0576

Author

Grimm, Daniela ; Infanger, Manfred ; Westphal, Kris ; Ulbrich, Claudia ; Pietsch, Jessica ; Kossmehl, Peter ; Vadrucci, Sonia ; Baatout, Sarah ; Flick, Burkhard ; Paul, Martin ; Bauer, Johann ; Beck, Michaël ; Ghardi, Myriam. / A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness. In: Tissue Engineering Part A. 2009 ; Vol. 15, No. 8. pp. 2267-2275.

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@article{a780805eb1b64957a5786a9126efc1ba,
title = "A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness",
abstract = "Endothelial cells (EC) form three-dimensional aggregates without any scaffold, when they are exposed to microgravity (µg) simulated by a random positioning machine (RPM), but not under static 1g conditions. Here we describe a delayed type of formation of 3D structures of EC, which was initiated, when EC cultured on a Desktop-RPM remained adherent for the first five days, but spread over neighboring adherent cells forming little colonies. After two weeks, tube-like structures (TS) became visible in these cultures. They included a lumen and elongated during another two weeks of culturing. The walls of these TS consisted mainly of single-layered EC, which had produced significantly more 1-integrin, laminin, fibronectin, and alpha-tubulin than EC simultaneously grown adhering to the culture dishes under µg or under normal gravity. The amount of actin protein was similar in EC incorporated in TS and in EC growing at 1g. Interestingly, the ratio of TIMP-1 to MMP-2 found in the supernatants was lower at the seventh than at the 28th day of culturing. These results suggest that culturing EC under conditions of modeled gravitational unloading represents a new technique to study the formation of tubes which resemble vascular intimas.",
keywords = "weightlessness, endothelial cells, space, human",
author = "Daniela Grimm and Manfred Infanger and Kris Westphal and Claudia Ulbrich and Jessica Pietsch and Peter Kossmehl and Sonia Vadrucci and Sarah Baatout and Burkhard Flick and Martin Paul and Johann Bauer and Micha{\"e}l Beck and Myriam Ghardi",
note = "Score = 10",
year = "2009",
month = "2",
day = "3",
doi = "10.1089/ten.tea.2008.0576",
language = "English",
volume = "15",
pages = "2267--2275",
journal = "Tissue Engineering Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

RIS - Download

TY - JOUR

T1 - A Delayed Type of Three-Dimensional Growth of Human Endothelial Cells under Simulated Weightlessness

AU - Grimm, Daniela

AU - Infanger, Manfred

AU - Westphal, Kris

AU - Ulbrich, Claudia

AU - Pietsch, Jessica

AU - Kossmehl, Peter

AU - Vadrucci, Sonia

AU - Baatout, Sarah

AU - Flick, Burkhard

AU - Paul, Martin

AU - Bauer, Johann

A2 - Beck, Michaël

A2 - Ghardi, Myriam

N1 - Score = 10

PY - 2009/2/3

Y1 - 2009/2/3

N2 - Endothelial cells (EC) form three-dimensional aggregates without any scaffold, when they are exposed to microgravity (µg) simulated by a random positioning machine (RPM), but not under static 1g conditions. Here we describe a delayed type of formation of 3D structures of EC, which was initiated, when EC cultured on a Desktop-RPM remained adherent for the first five days, but spread over neighboring adherent cells forming little colonies. After two weeks, tube-like structures (TS) became visible in these cultures. They included a lumen and elongated during another two weeks of culturing. The walls of these TS consisted mainly of single-layered EC, which had produced significantly more 1-integrin, laminin, fibronectin, and alpha-tubulin than EC simultaneously grown adhering to the culture dishes under µg or under normal gravity. The amount of actin protein was similar in EC incorporated in TS and in EC growing at 1g. Interestingly, the ratio of TIMP-1 to MMP-2 found in the supernatants was lower at the seventh than at the 28th day of culturing. These results suggest that culturing EC under conditions of modeled gravitational unloading represents a new technique to study the formation of tubes which resemble vascular intimas.

AB - Endothelial cells (EC) form three-dimensional aggregates without any scaffold, when they are exposed to microgravity (µg) simulated by a random positioning machine (RPM), but not under static 1g conditions. Here we describe a delayed type of formation of 3D structures of EC, which was initiated, when EC cultured on a Desktop-RPM remained adherent for the first five days, but spread over neighboring adherent cells forming little colonies. After two weeks, tube-like structures (TS) became visible in these cultures. They included a lumen and elongated during another two weeks of culturing. The walls of these TS consisted mainly of single-layered EC, which had produced significantly more 1-integrin, laminin, fibronectin, and alpha-tubulin than EC simultaneously grown adhering to the culture dishes under µg or under normal gravity. The amount of actin protein was similar in EC incorporated in TS and in EC growing at 1g. Interestingly, the ratio of TIMP-1 to MMP-2 found in the supernatants was lower at the seventh than at the 28th day of culturing. These results suggest that culturing EC under conditions of modeled gravitational unloading represents a new technique to study the formation of tubes which resemble vascular intimas.

KW - weightlessness

KW - endothelial cells

KW - space

KW - human

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_94533

UR - http://knowledgecentre.sckcen.be/so2/bibref/5532

U2 - 10.1089/ten.tea.2008.0576

DO - 10.1089/ten.tea.2008.0576

M3 - Article

VL - 15

SP - 2267

EP - 2275

JO - Tissue Engineering Part A

JF - Tissue Engineering Part A

SN - 1937-3341

IS - 8

ER -

ID: 238713