A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics

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A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics. / Masschelein, Joleen; Mattheus, Wesley; Gao, Ling-Jie; Moons, Pieter; Van Houdt, Rob; Uytterhoeven, Birgit; Lamberigts, Chris; Lescinier, Eveline; Rozenski, Jef; Herdewijn, Piet; Aertsen, Abram; Michiels, Chris; Lavigne, Rob.

In: PLOS ONE, Vol. 8, No. 1, 17.01.2013, p. 54143-54143.

Research output: Contribution to journalArticlepeer-review

Harvard

Masschelein, J, Mattheus, W, Gao, L-J, Moons, P, Van Houdt, R, Uytterhoeven, B, Lamberigts, C, Lescinier, E, Rozenski, J, Herdewijn, P, Aertsen, A, Michiels, C & Lavigne, R 2013, 'A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics', PLOS ONE, vol. 8, no. 1, pp. 54143-54143. https://doi.org/10.1371/journal.pone.0054143

APA

Masschelein, J., Mattheus, W., Gao, L-J., Moons, P., Van Houdt, R., Uytterhoeven, B., Lamberigts, C., Lescinier, E., Rozenski, J., Herdewijn, P., Aertsen, A., Michiels, C., & Lavigne, R. (2013). A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics. PLOS ONE, 8(1), 54143-54143. https://doi.org/10.1371/journal.pone.0054143

Author

Masschelein, Joleen ; Mattheus, Wesley ; Gao, Ling-Jie ; Moons, Pieter ; Van Houdt, Rob ; Uytterhoeven, Birgit ; Lamberigts, Chris ; Lescinier, Eveline ; Rozenski, Jef ; Herdewijn, Piet ; Aertsen, Abram ; Michiels, Chris ; Lavigne, Rob. / A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics. In: PLOS ONE. 2013 ; Vol. 8, No. 1. pp. 54143-54143.

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@article{c885e5b94c464b9cab12428decabdfbe,
title = "A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics",
abstract = "Serratia plymuthica strain RVH1, initially isolated from an industrial food processing environment, displays potent antimicrobial activity towards a broad spectrum of Gram-positive and Gram-negative bacterial pathogens. Isolation and subsequent structure determination of bioactive molecules led to the identification of two polyamino antibiotics with the same molecular structure as zeamine and zeamine II as well as a third, closely related analogue, designated zeamine I. The gene cluster encoding the biosynthesis of the zeamine antibiotics was cloned and sequenced and shown to encode FAS, PKS as well as NRPS related enzymes in addition to putative tailoring and export enzymes. Interestingly, several genes show strong homology to the pfa cluster of genes involved in the biosynthesis of long chain polyunsaturated fatty acids in marine bacteria. We postulate that a mixed FAS/PKS and a hybrid NRPS/PKS assembly line each synthesize parts of the backbone that are linked together post-assembly in the case of zeamine and zeamine I. This interaction reflects a unique interplay between secondary lipid and secondary metabolite biosynthesis. Most likely, the zeamine antibiotics are produced as prodrugs that undergo activation in which a nonribosomal peptide sequence is cleaved off.",
keywords = "antibiotics, quorum sensing",
author = "Joleen Masschelein and Wesley Mattheus and Ling-Jie Gao and Pieter Moons and {Van Houdt}, Rob and Birgit Uytterhoeven and Chris Lamberigts and Eveline Lescinier and Jef Rozenski and Piet Herdewijn and Abram Aertsen and Chris Michiels and Rob Lavigne",
note = "Score = 10",
year = "2013",
month = jan,
day = "17",
doi = "10.1371/journal.pone.0054143",
language = "English",
volume = "8",
pages = "54143--54143",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "PLOS - Public Library of Science",
number = "1",

}

RIS - Download

TY - JOUR

T1 - A PKS/NRPS/FAS Hybrid Gene Cluster from Serratia plymuthica RVH1 Encoding the Biosynthesis of Three Broad Spectrum, Zeamine-Related Antibiotics

AU - Masschelein, Joleen

AU - Mattheus, Wesley

AU - Gao, Ling-Jie

AU - Moons, Pieter

AU - Van Houdt, Rob

AU - Uytterhoeven, Birgit

AU - Lamberigts, Chris

AU - Lescinier, Eveline

AU - Rozenski, Jef

AU - Herdewijn, Piet

AU - Aertsen, Abram

AU - Michiels, Chris

AU - Lavigne, Rob

N1 - Score = 10

PY - 2013/1/17

Y1 - 2013/1/17

N2 - Serratia plymuthica strain RVH1, initially isolated from an industrial food processing environment, displays potent antimicrobial activity towards a broad spectrum of Gram-positive and Gram-negative bacterial pathogens. Isolation and subsequent structure determination of bioactive molecules led to the identification of two polyamino antibiotics with the same molecular structure as zeamine and zeamine II as well as a third, closely related analogue, designated zeamine I. The gene cluster encoding the biosynthesis of the zeamine antibiotics was cloned and sequenced and shown to encode FAS, PKS as well as NRPS related enzymes in addition to putative tailoring and export enzymes. Interestingly, several genes show strong homology to the pfa cluster of genes involved in the biosynthesis of long chain polyunsaturated fatty acids in marine bacteria. We postulate that a mixed FAS/PKS and a hybrid NRPS/PKS assembly line each synthesize parts of the backbone that are linked together post-assembly in the case of zeamine and zeamine I. This interaction reflects a unique interplay between secondary lipid and secondary metabolite biosynthesis. Most likely, the zeamine antibiotics are produced as prodrugs that undergo activation in which a nonribosomal peptide sequence is cleaved off.

AB - Serratia plymuthica strain RVH1, initially isolated from an industrial food processing environment, displays potent antimicrobial activity towards a broad spectrum of Gram-positive and Gram-negative bacterial pathogens. Isolation and subsequent structure determination of bioactive molecules led to the identification of two polyamino antibiotics with the same molecular structure as zeamine and zeamine II as well as a third, closely related analogue, designated zeamine I. The gene cluster encoding the biosynthesis of the zeamine antibiotics was cloned and sequenced and shown to encode FAS, PKS as well as NRPS related enzymes in addition to putative tailoring and export enzymes. Interestingly, several genes show strong homology to the pfa cluster of genes involved in the biosynthesis of long chain polyunsaturated fatty acids in marine bacteria. We postulate that a mixed FAS/PKS and a hybrid NRPS/PKS assembly line each synthesize parts of the backbone that are linked together post-assembly in the case of zeamine and zeamine I. This interaction reflects a unique interplay between secondary lipid and secondary metabolite biosynthesis. Most likely, the zeamine antibiotics are produced as prodrugs that undergo activation in which a nonribosomal peptide sequence is cleaved off.

KW - antibiotics

KW - quorum sensing

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_127010

UR - http://knowledgecentre.sckcen.be/so2/bibref/9961

U2 - 10.1371/journal.pone.0054143

DO - 10.1371/journal.pone.0054143

M3 - Article

VL - 8

SP - 54143

EP - 54143

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 1

ER -

ID: 196356