A probable link between the DedA protein and resistance to selenite

Research output: Contribution to journalArticle

Authors

  • Max Mergeay
  • Fouzia LEDGHAM
  • Benjamin QUEST
  • Tatiana Vallaeys
  • Jacques COVES

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Abstract

To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while theMIC for the wild-type strain was estimated as 4–6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

Details

Original languageEnglish
Pages (from-to)367-374
JournalResearch in Microbiology
Volume156
StatePublished - Feb 2005

Keywords

  • Selenium oxyanions, Selenite, Resistance, Random mutagenesis, DedA, Ralstonia metallidurans CH34

ID: 166348