A probable link between the DedA protein and resistance to selenite

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A probable link between the DedA protein and resistance to selenite. / Mergeay, Max; LEDGHAM, Fouzia; QUEST, Benjamin; Vallaeys, Tatiana; COVES, Jacques.

In: Research in Microbiology, Vol. 156, 02.2005, p. 367-374.

Research output: Contribution to journalArticle

Harvard

Mergeay, M, LEDGHAM, F, QUEST, B, Vallaeys, T & COVES, J 2005, 'A probable link between the DedA protein and resistance to selenite', Research in Microbiology, vol. 156, pp. 367-374.

APA

Mergeay, M., LEDGHAM, F., QUEST, B., Vallaeys, T., & COVES, J. (2005). A probable link between the DedA protein and resistance to selenite. Research in Microbiology, 156, 367-374.

Vancouver

Mergeay M, LEDGHAM F, QUEST B, Vallaeys T, COVES J. A probable link between the DedA protein and resistance to selenite. Research in Microbiology. 2005 Feb;156:367-374.

Author

Mergeay, Max ; LEDGHAM, Fouzia ; QUEST, Benjamin ; Vallaeys, Tatiana ; COVES, Jacques. / A probable link between the DedA protein and resistance to selenite. In: Research in Microbiology. 2005 ; Vol. 156. pp. 367-374.

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@article{2edbf6817952462682d1bd275fd2e878,
title = "A probable link between the DedA protein and resistance to selenite",
abstract = "To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while theMIC for the wild-type strain was estimated as 4–6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.",
keywords = "Selenium oxyanions, Selenite, Resistance, Random mutagenesis, DedA, Ralstonia metallidurans CH34",
author = "Max Mergeay and Fouzia LEDGHAM and Benjamin QUEST and Tatiana Vallaeys and Jacques COVES",
note = "Score = 10",
year = "2005",
month = "2",
language = "English",
volume = "156",
pages = "367--374",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier",

}

RIS - Download

TY - JOUR

T1 - A probable link between the DedA protein and resistance to selenite

AU - Mergeay, Max

AU - LEDGHAM, Fouzia

AU - QUEST, Benjamin

AU - Vallaeys, Tatiana

AU - COVES, Jacques

N1 - Score = 10

PY - 2005/2

Y1 - 2005/2

N2 - To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while theMIC for the wild-type strain was estimated as 4–6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

AB - To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while theMIC for the wild-type strain was estimated as 4–6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

KW - Selenium oxyanions

KW - Selenite

KW - Resistance

KW - Random mutagenesis

KW - DedA

KW - Ralstonia metallidurans CH34

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_27248

UR - http://knowledgecentre.sckcen.be/so2/bibref/3089

M3 - Article

VL - 156

SP - 367

EP - 374

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

ER -

ID: 166348