Absolute quantification of microbial taxon abundances.

Research output: Contribution to journalArticle

Standard

Absolute quantification of microbial taxon abundances. / Props, Ruben; Monsieurs, Pieter; Kerckhof, FM; Rubbens, P; De, Vrieze J; Hernandez, Sanabria E; Waegeman, J; Hammes, F; Boon, N.

In: The ISME Journal: Multidisciplinary Journal of Microbial Ecology, Vol. 11, No. February 2017, 01.02.2017, p. 584-587.

Research output: Contribution to journalArticle

Harvard

Props, R, Monsieurs, P, Kerckhof, FM, Rubbens, P, De, VJ, Hernandez, SE, Waegeman, J, Hammes, F & Boon, N 2017, 'Absolute quantification of microbial taxon abundances.', The ISME Journal: Multidisciplinary Journal of Microbial Ecology, vol. 11, no. February 2017, pp. 584-587. https://doi.org/10.1038/ismej.2016.117

APA

Props, R., Monsieurs, P., Kerckhof, FM., Rubbens, P., De, V. J., Hernandez, S. E., ... Boon, N. (2017). Absolute quantification of microbial taxon abundances. The ISME Journal: Multidisciplinary Journal of Microbial Ecology, 11(February 2017), 584-587. https://doi.org/10.1038/ismej.2016.117

Vancouver

Props R, Monsieurs P, Kerckhof FM, Rubbens P, De VJ, Hernandez SE et al. Absolute quantification of microbial taxon abundances. The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 2017 Feb 1;11(February 2017):584-587. https://doi.org/10.1038/ismej.2016.117

Author

Props, Ruben ; Monsieurs, Pieter ; Kerckhof, FM ; Rubbens, P ; De, Vrieze J ; Hernandez, Sanabria E ; Waegeman, J ; Hammes, F ; Boon, N. / Absolute quantification of microbial taxon abundances. In: The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 2017 ; Vol. 11, No. February 2017. pp. 584-587.

Bibtex - Download

@article{a80c0b4d79ef4bf692fa4befa0158d78,
title = "Absolute quantification of microbial taxon abundances.",
abstract = "High-throughput amplicon sequencing has become a well-established approach for microbial community profiling. Correlating shifts in the relative abundances of bacterial taxa with environmental gradients is the goal of many microbiome surveys. As the abundances generated by this technology are semi-quantitative by definition, the observed dynamics may not accurately reflect those of the actual taxon densities. We combined the sequencing approach (16S rRNA gene) with robust single-cell enumeration technologies (flow cytometry) to quantify the absolute taxon abundances. A detailed longitudinal analysis of the absolute abundances resulted in distinct abundance profiles that were less ambiguous and expressed in units that can be directly compared across studies. We further provide evidence that the enrichment of taxa (increase in relative abundance) does not necessarily relate to the outgrowth of taxa (increase in absolute abundance). Our results highlight that both relative and absolute abundances should be considered for a comprehensive biological interpretation of microbiome surveys.",
keywords = "flow cytometry, bioinformatics, microbial ecology, Nuclear Installations",
author = "Ruben Props and Pieter Monsieurs and FM Kerckhof and P Rubbens and De, {Vrieze J} and Hernandez, {Sanabria E} and J Waegeman and F Hammes and N Boon",
note = "Score=10",
year = "2017",
month = "2",
day = "1",
doi = "10.1038/ismej.2016.117",
language = "English",
volume = "11",
pages = "584--587",
journal = "The ISME Journal: Multidisciplinary Journal of Microbial Ecology",
issn = "1751-7362",
publisher = "Nature Publishing Group",
number = "February 2017",

}

RIS - Download

TY - JOUR

T1 - Absolute quantification of microbial taxon abundances.

AU - Props, Ruben

AU - Monsieurs, Pieter

AU - Kerckhof, FM

AU - Rubbens, P

AU - De, Vrieze J

AU - Hernandez, Sanabria E

AU - Waegeman, J

AU - Hammes, F

AU - Boon, N

N1 - Score=10

PY - 2017/2/1

Y1 - 2017/2/1

N2 - High-throughput amplicon sequencing has become a well-established approach for microbial community profiling. Correlating shifts in the relative abundances of bacterial taxa with environmental gradients is the goal of many microbiome surveys. As the abundances generated by this technology are semi-quantitative by definition, the observed dynamics may not accurately reflect those of the actual taxon densities. We combined the sequencing approach (16S rRNA gene) with robust single-cell enumeration technologies (flow cytometry) to quantify the absolute taxon abundances. A detailed longitudinal analysis of the absolute abundances resulted in distinct abundance profiles that were less ambiguous and expressed in units that can be directly compared across studies. We further provide evidence that the enrichment of taxa (increase in relative abundance) does not necessarily relate to the outgrowth of taxa (increase in absolute abundance). Our results highlight that both relative and absolute abundances should be considered for a comprehensive biological interpretation of microbiome surveys.

AB - High-throughput amplicon sequencing has become a well-established approach for microbial community profiling. Correlating shifts in the relative abundances of bacterial taxa with environmental gradients is the goal of many microbiome surveys. As the abundances generated by this technology are semi-quantitative by definition, the observed dynamics may not accurately reflect those of the actual taxon densities. We combined the sequencing approach (16S rRNA gene) with robust single-cell enumeration technologies (flow cytometry) to quantify the absolute taxon abundances. A detailed longitudinal analysis of the absolute abundances resulted in distinct abundance profiles that were less ambiguous and expressed in units that can be directly compared across studies. We further provide evidence that the enrichment of taxa (increase in relative abundance) does not necessarily relate to the outgrowth of taxa (increase in absolute abundance). Our results highlight that both relative and absolute abundances should be considered for a comprehensive biological interpretation of microbiome surveys.

KW - flow cytometry

KW - bioinformatics

KW - microbial ecology

KW - Nuclear Installations

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/21585979

U2 - 10.1038/ismej.2016.117

DO - 10.1038/ismej.2016.117

M3 - Article

VL - 11

SP - 584

EP - 587

JO - The ISME Journal: Multidisciplinary Journal of Microbial Ecology

JF - The ISME Journal: Multidisciplinary Journal of Microbial Ecology

SN - 1751-7362

IS - February 2017

ER -

ID: 2059513