Adaptive silver resistance in Cupriavidus metallidurans

Research output: ThesisDoctoral thesis

Standard

Adaptive silver resistance in Cupriavidus metallidurans. / Mijnendonckx, Kristel; Leys, Natalie (Peer reviewer).

Louvain-la-Neuve, Belgium : UCL - Université Catholique de Louvain, 2013. 258 p.

Research output: ThesisDoctoral thesis

Harvard

Mijnendonckx, K & Leys, N 2013, 'Adaptive silver resistance in Cupriavidus metallidurans', UCL - Université catholique de Louvain , Louvain-la-Neuve, Belgium.

APA

Mijnendonckx, K., & Leys, N. (2013). Adaptive silver resistance in Cupriavidus metallidurans. Louvain-la-Neuve, Belgium: UCL - Université Catholique de Louvain.

Vancouver

Mijnendonckx K, Leys N. Adaptive silver resistance in Cupriavidus metallidurans. Louvain-la-Neuve, Belgium: UCL - Université Catholique de Louvain, 2013. 258 p.

Author

Mijnendonckx, Kristel ; Leys, Natalie. / Adaptive silver resistance in Cupriavidus metallidurans. Louvain-la-Neuve, Belgium : UCL - Université Catholique de Louvain, 2013. 258 p.

Bibtex - Download

@phdthesis{642fccf2b0fa4c49a0edc5e9f136bb57,
title = "Adaptive silver resistance in Cupriavidus metallidurans",
abstract = "Assessing and controlling microbial contamination during long-term manned spaceflight is of paramount importance as contamination can cause problems for the astronaut’s health and the infrastructure of the spacecraft. Cupriavidus and Ralstonia genera were identified and isolated during numerous monitoring campaigns from different space-related environments. This study aimed to gather more insights in the ability of these strains to thrive in these environments. All isolates acquired moderate to high tolerance against several stressors and can grow in oligotrophic conditions, enabling them to persist in the ISS and related environments in spite of the implemented disinfection and sterilization procedures. Silver resistance mechanisms were studied, as silver is used to sanitize water sources in space. All isolates tolerated silver concentrations higher than those measured in the drinking water aboard the ISS, and survived a 23-month exposure to silver in drinking water. Rapid evolution towards increased silver resistance was observed in C. metallidurans strains.The identified silver efflux pumps did not participate in this response. By contrast, the agrS gene coding for the histidine kinase was affected by mutation, resulting in the increased expression of the response regulator AgrR. AgrR cross-activated the expression of mmmQ, resulting in the increased silver resistance.",
keywords = "Cupriavidus, Ralstonia, IS element, silver, MGE, plasmid",
author = "Kristel Mijnendonckx and Natalie Leys",
note = "Score = 30",
year = "2013",
month = "11",
language = "English",
publisher = "UCL - Universit{\'e} Catholique de Louvain",
school = "UCL - Universit{\'e} catholique de Louvain",

}

RIS - Download

TY - THES

T1 - Adaptive silver resistance in Cupriavidus metallidurans

AU - Mijnendonckx, Kristel

A2 - Leys, Natalie

N1 - Score = 30

PY - 2013/11

Y1 - 2013/11

N2 - Assessing and controlling microbial contamination during long-term manned spaceflight is of paramount importance as contamination can cause problems for the astronaut’s health and the infrastructure of the spacecraft. Cupriavidus and Ralstonia genera were identified and isolated during numerous monitoring campaigns from different space-related environments. This study aimed to gather more insights in the ability of these strains to thrive in these environments. All isolates acquired moderate to high tolerance against several stressors and can grow in oligotrophic conditions, enabling them to persist in the ISS and related environments in spite of the implemented disinfection and sterilization procedures. Silver resistance mechanisms were studied, as silver is used to sanitize water sources in space. All isolates tolerated silver concentrations higher than those measured in the drinking water aboard the ISS, and survived a 23-month exposure to silver in drinking water. Rapid evolution towards increased silver resistance was observed in C. metallidurans strains.The identified silver efflux pumps did not participate in this response. By contrast, the agrS gene coding for the histidine kinase was affected by mutation, resulting in the increased expression of the response regulator AgrR. AgrR cross-activated the expression of mmmQ, resulting in the increased silver resistance.

AB - Assessing and controlling microbial contamination during long-term manned spaceflight is of paramount importance as contamination can cause problems for the astronaut’s health and the infrastructure of the spacecraft. Cupriavidus and Ralstonia genera were identified and isolated during numerous monitoring campaigns from different space-related environments. This study aimed to gather more insights in the ability of these strains to thrive in these environments. All isolates acquired moderate to high tolerance against several stressors and can grow in oligotrophic conditions, enabling them to persist in the ISS and related environments in spite of the implemented disinfection and sterilization procedures. Silver resistance mechanisms were studied, as silver is used to sanitize water sources in space. All isolates tolerated silver concentrations higher than those measured in the drinking water aboard the ISS, and survived a 23-month exposure to silver in drinking water. Rapid evolution towards increased silver resistance was observed in C. metallidurans strains.The identified silver efflux pumps did not participate in this response. By contrast, the agrS gene coding for the histidine kinase was affected by mutation, resulting in the increased expression of the response regulator AgrR. AgrR cross-activated the expression of mmmQ, resulting in the increased silver resistance.

KW - Cupriavidus

KW - Ralstonia

KW - IS element

KW - silver

KW - MGE

KW - plasmid

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131712

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131712_2

M3 - Doctoral thesis

PB - UCL - Université Catholique de Louvain

CY - Louvain-la-Neuve, Belgium

ER -

ID: 76760