Age-dependent differences in DNA damage after in vitro CT exposure

Research output: Contribution to journalArticle

Authors

  • Maria Gomolka
  • Ursula Oestreicher
  • Ute Roessler
  • Daniel Samaga
  • David Endesfelder
  • Peter Lang
  • Klement Neumaier
  • Claus Belka
  • Marion Kiechle
  • Markus Niemeyer
  • Uwe Hasbargen
  • Christoph Hoeubener
  • Hans-Joachim Kirlum
  • Ulrike Kulka
  • Albert Rosenberger
  • Linda Walsh
  • Sarah Baatout
  • Ausrele Kesminiene
  • Carita Lindholm

Institutes & Expert groups

  • Bfs - Bundesamt Für Strahlenschutz - Federal Office for Radiation Protection
  • LMU Munich - Ludwig-Maximilians-Universitat Munchen
  • TUM - Technical University of Munich
  • Praxis für Kinderchirurgie
  • Universitätsmedizin Göttingen - Institut für Allgemeinmedizin
  • IARC - International Agency for Research on Cancer
  • STUK - Radiation and Nuclear Safety Authority - Finland

Documents & links

Abstract

Purpose: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. Materials and methods: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under five years and adults after sham exposure (0 mGy), low-dose (41 mGy) and high-dose (978 mGy) in vitro CT exposure were analyzed. Results: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1.48 (95% CI 1.30–1.68), after exposure to 978 mGy. Although a significant dose response for damage induction and dose-dependent repair was found, the gammaH2AX assay did not show an age-dependent increase in DNA damage in newborns/ children compared to adults. This was the case for the gammaH2AX levels after repair time intervals of 30 minutes and 24 hours, after correcting for the underlying background damage. For the low dose of 41 mGy, the power of the dicentric assay was also not sufficient to detect an age-dependent effect in the sample size investigated. Conclusion: A 1.5-fold increased level of dicentric aberrations is detected in newborns and children under five years after 1 Gy radiation exposure.

Details

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalInternational Journal of Radiation Biology
Volume94(3)
Issue number272-281
DOIs
Publication statusPublished - 25 Jan 2018

Keywords

  • Computed tomography, age-related radiation sensitivity, age, gender related radiation sensitivity, gender, cancer, trauma, DNA damage, gamma H2AX

ID: 4789383