Age-dependent differences in DNA damage after in vitro CT exposure

Research output: Contribution to journalArticlepeer-review

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Age-dependent differences in DNA damage after in vitro CT exposure. / Gomolka, Maria; Oestreicher, Ursula; Roessler, Ute; Samaga, Daniel; Endesfelder, David; Lang, Peter; Neumaier, Klement; Belka, Claus; Kiechle, Marion; Niemeyer, Markus; Hasbargen, Uwe; Hoeubener, Christoph; Kirlum, Hans-Joachim; Kulka, Ulrike; Rosenberger, Albert; Walsh, Linda; Baatout, Sarah; Kesminiene, Ausrele; Lindholm, Carita.

In: International Journal of Radiation Biology, Vol. 94(3), No. 272-281, 25.01.2018, p. 272-281.

Research output: Contribution to journalArticlepeer-review

Harvard

Gomolka, M, Oestreicher, U, Roessler, U, Samaga, D, Endesfelder, D, Lang, P, Neumaier, K, Belka, C, Kiechle, M, Niemeyer, M, Hasbargen, U, Hoeubener, C, Kirlum, H-J, Kulka, U, Rosenberger, A, Walsh, L, Baatout, S, Kesminiene, A & Lindholm, C 2018, 'Age-dependent differences in DNA damage after in vitro CT exposure', International Journal of Radiation Biology, vol. 94(3), no. 272-281, pp. 272-281. https://doi.org/10.1080/09553002.2018.1419302

APA

Gomolka, M., Oestreicher, U., Roessler, U., Samaga, D., Endesfelder, D., Lang, P., Neumaier, K., Belka, C., Kiechle, M., Niemeyer, M., Hasbargen, U., Hoeubener, C., Kirlum, H-J., Kulka, U., Rosenberger, A., Walsh, L., Baatout, S., Kesminiene, A., & Lindholm, C. (2018). Age-dependent differences in DNA damage after in vitro CT exposure. International Journal of Radiation Biology, 94(3)(272-281), 272-281. https://doi.org/10.1080/09553002.2018.1419302

Vancouver

Gomolka M, Oestreicher U, Roessler U, Samaga D, Endesfelder D, Lang P et al. Age-dependent differences in DNA damage after in vitro CT exposure. International Journal of Radiation Biology. 2018 Jan 25;94(3)(272-281):272-281. https://doi.org/10.1080/09553002.2018.1419302

Author

Gomolka, Maria ; Oestreicher, Ursula ; Roessler, Ute ; Samaga, Daniel ; Endesfelder, David ; Lang, Peter ; Neumaier, Klement ; Belka, Claus ; Kiechle, Marion ; Niemeyer, Markus ; Hasbargen, Uwe ; Hoeubener, Christoph ; Kirlum, Hans-Joachim ; Kulka, Ulrike ; Rosenberger, Albert ; Walsh, Linda ; Baatout, Sarah ; Kesminiene, Ausrele ; Lindholm, Carita. / Age-dependent differences in DNA damage after in vitro CT exposure. In: International Journal of Radiation Biology. 2018 ; Vol. 94(3), No. 272-281. pp. 272-281.

Bibtex - Download

@article{8f09c480b23b489488c775192533d413,
title = "Age-dependent differences in DNA damage after in vitro CT exposure",
abstract = "Purpose: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. Materials and methods: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under five years and adults after sham exposure (0 mGy), low-dose (41 mGy) and high-dose (978 mGy) in vitro CT exposure were analyzed. Results: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1.48 (95% CI 1.30–1.68), after exposure to 978 mGy. Although a significant dose response for damage induction and dose-dependent repair was found, the gammaH2AX assay did not show an age-dependent increase in DNA damage in newborns/ children compared to adults. This was the case for the gammaH2AX levels after repair time intervals of 30 minutes and 24 hours, after correcting for the underlying background damage. For the low dose of 41 mGy, the power of the dicentric assay was also not sufficient to detect an age-dependent effect in the sample size investigated. Conclusion: A 1.5-fold increased level of dicentric aberrations is detected in newborns and children under five years after 1 Gy radiation exposure.",
keywords = "Computed tomography, age-related radiation sensitivity, age, gender related radiation sensitivity, gender, cancer, trauma, DNA damage, gamma H2AX",
author = "Maria Gomolka and Ursula Oestreicher and Ute Roessler and Daniel Samaga and David Endesfelder and Peter Lang and Klement Neumaier and Claus Belka and Marion Kiechle and Markus Niemeyer and Uwe Hasbargen and Christoph Hoeubener and Hans-Joachim Kirlum and Ulrike Kulka and Albert Rosenberger and Linda Walsh and Sarah Baatout and Ausrele Kesminiene and Carita Lindholm",
note = "Score=10",
year = "2018",
month = jan,
day = "25",
doi = "10.1080/09553002.2018.1419302",
language = "English",
volume = "94(3)",
pages = "272--281",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Taylor & Francis (CRC)",
number = "272-281",

}

RIS - Download

TY - JOUR

T1 - Age-dependent differences in DNA damage after in vitro CT exposure

AU - Gomolka, Maria

AU - Oestreicher, Ursula

AU - Roessler, Ute

AU - Samaga, Daniel

AU - Endesfelder, David

AU - Lang, Peter

AU - Neumaier, Klement

AU - Belka, Claus

AU - Kiechle, Marion

AU - Niemeyer, Markus

AU - Hasbargen, Uwe

AU - Hoeubener, Christoph

AU - Kirlum, Hans-Joachim

AU - Kulka, Ulrike

AU - Rosenberger, Albert

AU - Walsh, Linda

AU - Baatout, Sarah

AU - Kesminiene, Ausrele

AU - Lindholm, Carita

N1 - Score=10

PY - 2018/1/25

Y1 - 2018/1/25

N2 - Purpose: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. Materials and methods: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under five years and adults after sham exposure (0 mGy), low-dose (41 mGy) and high-dose (978 mGy) in vitro CT exposure were analyzed. Results: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1.48 (95% CI 1.30–1.68), after exposure to 978 mGy. Although a significant dose response for damage induction and dose-dependent repair was found, the gammaH2AX assay did not show an age-dependent increase in DNA damage in newborns/ children compared to adults. This was the case for the gammaH2AX levels after repair time intervals of 30 minutes and 24 hours, after correcting for the underlying background damage. For the low dose of 41 mGy, the power of the dicentric assay was also not sufficient to detect an age-dependent effect in the sample size investigated. Conclusion: A 1.5-fold increased level of dicentric aberrations is detected in newborns and children under five years after 1 Gy radiation exposure.

AB - Purpose: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. Materials and methods: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under five years and adults after sham exposure (0 mGy), low-dose (41 mGy) and high-dose (978 mGy) in vitro CT exposure were analyzed. Results: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1.48 (95% CI 1.30–1.68), after exposure to 978 mGy. Although a significant dose response for damage induction and dose-dependent repair was found, the gammaH2AX assay did not show an age-dependent increase in DNA damage in newborns/ children compared to adults. This was the case for the gammaH2AX levels after repair time intervals of 30 minutes and 24 hours, after correcting for the underlying background damage. For the low dose of 41 mGy, the power of the dicentric assay was also not sufficient to detect an age-dependent effect in the sample size investigated. Conclusion: A 1.5-fold increased level of dicentric aberrations is detected in newborns and children under five years after 1 Gy radiation exposure.

KW - Computed tomography

KW - age-related radiation sensitivity

KW - age

KW - gender related radiation sensitivity

KW - gender

KW - cancer

KW - trauma

KW - DNA damage

KW - gamma H2AX

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/32401979

U2 - 10.1080/09553002.2018.1419302

DO - 10.1080/09553002.2018.1419302

M3 - Article

VL - 94(3)

SP - 272

EP - 281

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 272-281

ER -

ID: 4789383