An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira

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An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira. / Morin, Nicolas; Vallaeys, Tatiana; Hendrickx, Larissa; Leys, Natalie; Wilmotte, Annick.

In: Journal of Microbiological Methods, Vol. 80, No. 2, 04.01.2010, p. 148-154.

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Morin, Nicolas ; Vallaeys, Tatiana ; Hendrickx, Larissa ; Leys, Natalie ; Wilmotte, Annick. / An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira. In: Journal of Microbiological Methods. 2010 ; Vol. 80, No. 2. pp. 148-154.

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@article{ca88be7cbbc74784b866e3f3dacef2ae,
title = "An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira",
abstract = "Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria. In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.",
keywords = "An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira",
author = "Nicolas Morin and Tatiana Vallaeys and Larissa Hendrickx and Natalie Leys and Annick Wilmotte",
note = "Score = 10",
year = "2010",
month = jan,
day = "4",
doi = "10.1016/j.mimet.2009.11.012",
language = "English",
volume = "80",
pages = "148--154",
journal = "Journal of Microbiological Methods",
issn = "1872-8359",
publisher = "Elsevier",
number = "2",

}

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TY - JOUR

T1 - An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira

AU - Morin, Nicolas

AU - Vallaeys, Tatiana

AU - Hendrickx, Larissa

AU - Leys, Natalie

AU - Wilmotte, Annick

N1 - Score = 10

PY - 2010/1/4

Y1 - 2010/1/4

N2 - Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria. In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.

AB - Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria. In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.

KW - An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_103524

UR - http://knowledgecentre.sckcen.be/so2/bibref/6711

U2 - 10.1016/j.mimet.2009.11.012

DO - 10.1016/j.mimet.2009.11.012

M3 - Article

VL - 80

SP - 148

EP - 154

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 1872-8359

IS - 2

ER -

ID: 229798