Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum

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Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. / Crabbé, Aurélie; Liu, Yulong; Matthijs, Nele; Rigole, Petra; De La Fuente-Nùñez, César; Davis, Richard; Ledesma, Maria A.; Sarker, Shameema; Van Houdt, Rob; Hancock, Robert E.W.; Coenye, Tom; Nickerson, Cheryl A.

In: Scientific Reports, Vol. 7, 43321, 03.03.2017, p. 1-13.

Research output: Contribution to journalArticlepeer-review

Harvard

Crabbé, A, Liu, Y, Matthijs, N, Rigole, P, De La Fuente-Nùñez, C, Davis, R, Ledesma, MA, Sarker, S, Van Houdt, R, Hancock, REW, Coenye, T & Nickerson, CA 2017, 'Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum', Scientific Reports, vol. 7, 43321, pp. 1-13. https://doi.org/10.1038/srep43321

APA

Crabbé, A., Liu, Y., Matthijs, N., Rigole, P., De La Fuente-Nùñez, C., Davis, R., Ledesma, M. A., Sarker, S., Van Houdt, R., Hancock, R. E. W., Coenye, T., & Nickerson, C. A. (2017). Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. Scientific Reports, 7, 1-13. [43321]. https://doi.org/10.1038/srep43321

Vancouver

Crabbé A, Liu Y, Matthijs N, Rigole P, De La Fuente-Nùñez C, Davis R et al. Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. Scientific Reports. 2017 Mar 3;7:1-13. 43321. https://doi.org/10.1038/srep43321

Author

Crabbé, Aurélie ; Liu, Yulong ; Matthijs, Nele ; Rigole, Petra ; De La Fuente-Nùñez, César ; Davis, Richard ; Ledesma, Maria A. ; Sarker, Shameema ; Van Houdt, Rob ; Hancock, Robert E.W. ; Coenye, Tom ; Nickerson, Cheryl A. / Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. In: Scientific Reports. 2017 ; Vol. 7. pp. 1-13.

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@article{dfe6932694c64bd6b41c724d84dde683,
title = "Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum",
abstract = "In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. P. aeruginosa formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of P. aeruginosa association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against P. aeruginosa associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit P. aeruginosa association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against P. aeruginosa biofilm formation, which in turn depends on the presence or absence of FBS.",
keywords = "Pseudomonas, biofilm, antimicrobial activity, cystic fibrosis, 3-D model",
author = "Aur{\'e}lie Crabb{\'e} and Yulong Liu and Nele Matthijs and Petra Rigole and {De La Fuente-N{\`u}{\~n}ez}, C{\'e}sar and Richard Davis and Ledesma, {Maria A.} and Shameema Sarker and {Van Houdt}, Rob and Hancock, {Robert E.W.} and Tom Coenye and Nickerson, {Cheryl A.}",
note = "SCORE=10",
year = "2017",
month = mar,
day = "3",
doi = "10.1038/srep43321",
language = "English",
volume = "7",
pages = "1--13",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS - Download

TY - JOUR

T1 - Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum

AU - Crabbé, Aurélie

AU - Liu, Yulong

AU - Matthijs, Nele

AU - Rigole, Petra

AU - De La Fuente-Nùñez, César

AU - Davis, Richard

AU - Ledesma, Maria A.

AU - Sarker, Shameema

AU - Van Houdt, Rob

AU - Hancock, Robert E.W.

AU - Coenye, Tom

AU - Nickerson, Cheryl A.

N1 - SCORE=10

PY - 2017/3/3

Y1 - 2017/3/3

N2 - In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. P. aeruginosa formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of P. aeruginosa association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against P. aeruginosa associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit P. aeruginosa association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against P. aeruginosa biofilm formation, which in turn depends on the presence or absence of FBS.

AB - In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. P. aeruginosa formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of P. aeruginosa association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against P. aeruginosa associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit P. aeruginosa association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against P. aeruginosa biofilm formation, which in turn depends on the presence or absence of FBS.

KW - Pseudomonas

KW - biofilm

KW - antimicrobial activity

KW - cystic fibrosis

KW - 3-D model

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/22514206

U2 - 10.1038/srep43321

DO - 10.1038/srep43321

M3 - Article

VL - 7

SP - 1

EP - 13

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 43321

ER -

ID: 2224437