Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response

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@article{e528d679e0354d2a9d16d82a5eebded5,
title = "Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response",
abstract = "When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the P0 generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation.",
keywords = "Arabidopsis, gamma irradiation, multiple generations, long-term effects, oxidative stress",
author = "{van de Walle}, Jorden and Nele Horemans and Eline Saenen and {Van Hees}, May and Jean Wannijn and Robin Nauts and {Van Gompel}, Axel and Jaco Vangronsveld and Hildegarde Vandenhove and Ann Cuypers",
note = "Score=10",
year = "2016",
month = "12",
day = "21",
doi = "10.1016/j.jenvrad.2016.10.014",
language = "English",
volume = "165",
pages = "270--279",
journal = "Journal of environmental radioactivity",
issn = "0265-931X",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response

AU - van de Walle, Jorden

AU - Horemans, Nele

AU - Saenen, Eline

AU - Van Hees, May

AU - Wannijn, Jean

AU - Nauts, Robin

AU - Van Gompel, Axel

AU - Vangronsveld, Jaco

AU - Vandenhove, Hildegarde

AU - Cuypers, Ann

N1 - Score=10

PY - 2016/12/21

Y1 - 2016/12/21

N2 - When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the P0 generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation.

AB - When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the P0 generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation.

KW - Arabidopsis

KW - gamma irradiation

KW - multiple generations

KW - long-term effects

KW - oxidative stress

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/21989184

U2 - 10.1016/j.jenvrad.2016.10.014

DO - 10.1016/j.jenvrad.2016.10.014

M3 - Article

VL - 165

SP - 270

EP - 279

JO - Journal of environmental radioactivity

JF - Journal of environmental radioactivity

SN - 0265-931X

ER -

ID: 2153856