Bacillus thuringiensis conjugation in simulated microgravity

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Bacillus thuringiensis conjugation in simulated microgravity. / Beuls, Elise; Van Houdt, Rob; Leys, Natalie; Dijkstra, Camelia; Larkin, Oliver; Mahillon, Jacques; Mijnendonckx, Kristel (Peer reviewer).

In: Astrobiology, Vol. 9, No. 8, 21.10.2009, p. 797-805.

Research output: Contribution to journalArticle

Harvard

Beuls, E, Van Houdt, R, Leys, N, Dijkstra, C, Larkin, O, Mahillon, J & Mijnendonckx, K 2009, 'Bacillus thuringiensis conjugation in simulated microgravity', Astrobiology, vol. 9, no. 8, pp. 797-805. https://doi.org/10.1089/ast.2009.0383

Vancouver

Beuls E, Van Houdt R, Leys N, Dijkstra C, Larkin O, Mahillon J et al. Bacillus thuringiensis conjugation in simulated microgravity. Astrobiology. 2009 Oct 21;9(8):797-805. https://doi.org/10.1089/ast.2009.0383

Author

Beuls, Elise ; Van Houdt, Rob ; Leys, Natalie ; Dijkstra, Camelia ; Larkin, Oliver ; Mahillon, Jacques ; Mijnendonckx, Kristel. / Bacillus thuringiensis conjugation in simulated microgravity. In: Astrobiology. 2009 ; Vol. 9, No. 8. pp. 797-805.

Bibtex - Download

@article{abba24408a744170b20ee50ee9bdcfe2,
title = "Bacillus thuringiensis conjugation in simulated microgravity",
abstract = "Spaceflight experiments have suggested a possible effect of microgravity on the plasmid transfer among strains of the Gram-positive Bacillus thuringiensis, as opposed to no effect recorded for Gram-negative conjugation. To investigate these potential effects in a more affordable experimental setup, three ground-based microgravity simulators were tested: the Rotating Wall Vessel (RWV), the Random Positioning Machine (RPM), and a superconducting magnet. The bacterial conjugative system consisted in biparental matings between two B. thuringiensis strains, where the transfer frequencies of the conjugative plasmid pAW63 and its ability to mobilize the nonconjugative plasmid pUB110 were assessed. Specifically, potential plasmid transfers in a 0g position (simulated microgravity) were compared to those obtained under 1g (normal gravity) condition in each device. Statistical analyses revealed no significant difference in the conjugative and mobilizable transfer frequencies between the three different simulated microgravitational conditions and our standard laboratory condition. These important ground-based observations emphasize the fact that, though no stimulation of plasmid transfer was observed, no inhibition was observed either. In the case of Gram-positive bacteria, this ability to exchange plasmids in weightlessness, as occurs under Earth's conditions, should be seen as particularly relevant in the scope of spread of antibiotic resistances and bacterial virulence.",
keywords = "space, conjugation, plasmid, microgravity",
author = "Elise Beuls and {Van Houdt}, Rob and Natalie Leys and Camelia Dijkstra and Oliver Larkin and Jacques Mahillon and Kristel Mijnendonckx",
note = "Score = 10",
year = "2009",
month = "10",
day = "21",
doi = "10.1089/ast.2009.0383",
language = "English",
volume = "9",
pages = "797--805",
journal = "Astrobiology",
issn = "1531-1074",
publisher = "Mary Ann Liebert Inc. Publishers",
number = "8",

}

RIS - Download

TY - JOUR

T1 - Bacillus thuringiensis conjugation in simulated microgravity

AU - Beuls, Elise

AU - Van Houdt, Rob

AU - Leys, Natalie

AU - Dijkstra, Camelia

AU - Larkin, Oliver

AU - Mahillon, Jacques

A2 - Mijnendonckx, Kristel

N1 - Score = 10

PY - 2009/10/21

Y1 - 2009/10/21

N2 - Spaceflight experiments have suggested a possible effect of microgravity on the plasmid transfer among strains of the Gram-positive Bacillus thuringiensis, as opposed to no effect recorded for Gram-negative conjugation. To investigate these potential effects in a more affordable experimental setup, three ground-based microgravity simulators were tested: the Rotating Wall Vessel (RWV), the Random Positioning Machine (RPM), and a superconducting magnet. The bacterial conjugative system consisted in biparental matings between two B. thuringiensis strains, where the transfer frequencies of the conjugative plasmid pAW63 and its ability to mobilize the nonconjugative plasmid pUB110 were assessed. Specifically, potential plasmid transfers in a 0g position (simulated microgravity) were compared to those obtained under 1g (normal gravity) condition in each device. Statistical analyses revealed no significant difference in the conjugative and mobilizable transfer frequencies between the three different simulated microgravitational conditions and our standard laboratory condition. These important ground-based observations emphasize the fact that, though no stimulation of plasmid transfer was observed, no inhibition was observed either. In the case of Gram-positive bacteria, this ability to exchange plasmids in weightlessness, as occurs under Earth's conditions, should be seen as particularly relevant in the scope of spread of antibiotic resistances and bacterial virulence.

AB - Spaceflight experiments have suggested a possible effect of microgravity on the plasmid transfer among strains of the Gram-positive Bacillus thuringiensis, as opposed to no effect recorded for Gram-negative conjugation. To investigate these potential effects in a more affordable experimental setup, three ground-based microgravity simulators were tested: the Rotating Wall Vessel (RWV), the Random Positioning Machine (RPM), and a superconducting magnet. The bacterial conjugative system consisted in biparental matings between two B. thuringiensis strains, where the transfer frequencies of the conjugative plasmid pAW63 and its ability to mobilize the nonconjugative plasmid pUB110 were assessed. Specifically, potential plasmid transfers in a 0g position (simulated microgravity) were compared to those obtained under 1g (normal gravity) condition in each device. Statistical analyses revealed no significant difference in the conjugative and mobilizable transfer frequencies between the three different simulated microgravitational conditions and our standard laboratory condition. These important ground-based observations emphasize the fact that, though no stimulation of plasmid transfer was observed, no inhibition was observed either. In the case of Gram-positive bacteria, this ability to exchange plasmids in weightlessness, as occurs under Earth's conditions, should be seen as particularly relevant in the scope of spread of antibiotic resistances and bacterial virulence.

KW - space

KW - conjugation

KW - plasmid

KW - microgravity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_100344

UR - http://knowledgecentre.sckcen.be/so2/bibref/6089

U2 - 10.1089/ast.2009.0383

DO - 10.1089/ast.2009.0383

M3 - Article

VL - 9

SP - 797

EP - 805

JO - Astrobiology

JF - Astrobiology

SN - 1531-1074

IS - 8

ER -

ID: 289929