Biodistribution of Novel 68Ga-Radiolabelled HER2 Aptamers in Mice

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Abstract

Abstract Background: Two novel HER2 aptamers were recently selected with great potential for the in vitro diagnosis of HER2-positive cancer. The goal of this study was to examine the in vivo diagnostic potential of these HER2 aptamers. Methods: Both HER2 aptamers were radiolabelled with 68Ga, injected in mice bearing a HER2-positive and HER2-negative tumour and evaluated by PET/MRI. Results: Ex vivo bio distribution analysis revealed high uptake in the blood, tissues and organs, except the brain. Interestingly, this high uptake was explained by the slow blood clearance due to non-specific aptamer binding to blood proteins. We observed accumulation of radioactivity in both tumours in time. Although higher uptake in the HER2-positive tumour compared to the HER2-negative tumour was observed, this was accompanied with more necrosis in the HER2-negative tumour, which was observed by 18FDG PET/CT. Conclusion: This work presents a first step towards the development of 68Ga-labelled aptamers for molecular cancer imaging.

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Original languageEnglish
Article number1000300
Number of pages8
JournalJournal of Nuclear Medicine and Radiation Therapy
Volume7
Issue number5
DOIs
Publication statusPublished - 11 Aug 2016

Keywords

  • 68Ga, aptamers, Biodistribution, mice, HER2

ID: 2032096