Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide

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Authors

  • Catarina Xavier
  • Nick Devoogdt
  • Sophie Hernot
  • Ilse Vaneycken
  • Matthias D'huyvetter
  • Jens De Vos
  • Sam Massa
  • Tony Lahoutte
  • Vicky Caveliers

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Abstract

Chapter 30: the 99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.

Details

Original languageEnglish
Title of host publicationSinge Domain Antibodies, Methods and Protocols
Place of PublicationSpringer New York Heidelberg Dordrecht London, United States
PublisherSpringer
Pages485-490
Edition1
ISBN (Print)978-1-61779-968-6
StatePublished - Jan 2012

Publication series

NameMethods in Molecular Biology
Number911

Keywords

  • Nanobody, 99mTc

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