Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

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Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. / Beck, Michaël; Moreels, Marjan; Quintens, Roel; Abou-el-Ardat, Khalil; El Saghire, Houssein; Tabury, Kevin; Michaux, Arlette; Janssen, Ann; Neefs, Mieke; Van Oostveldt, Patrick; De Vos, Winnok H.; Baatout, Sarah.

In: International Journal of Molecular Medicine, Vol. 34, No. 2, 08.2014, p. 606-615.

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@article{20f46ba4d69e4192b5f526af88991aff,
title = "Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts",
abstract = "Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose (55 mSv) of ionizing radiation (IR), comprising a mixture of high linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibro¬blasts by inducing oxidative stress responsive genes. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.",
keywords = "simulated space conditions, microarrays, cytoskeleton, oxidative stress, DNA damage",
author = "Micha{\"e}l Beck and Marjan Moreels and Roel Quintens and Khalil Abou-el-Ardat and {El Saghire}, Houssein and Kevin Tabury and Arlette Michaux and Ann Janssen and Mieke Neefs and {Van Oostveldt}, Patrick and {De Vos}, {Winnok H.} and Sarah Baatout",
note = "Score = 10",
year = "2014",
month = "8",
doi = "10.3892/ijmm.2014.1785",
language = "English",
volume = "34",
pages = "606--615",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "2",

}

RIS - Download

TY - JOUR

T1 - Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

AU - Beck, Michaël

AU - Moreels, Marjan

AU - Quintens, Roel

AU - Abou-el-Ardat, Khalil

AU - El Saghire, Houssein

AU - Tabury, Kevin

AU - Michaux, Arlette

AU - Janssen, Ann

AU - Neefs, Mieke

AU - Van Oostveldt, Patrick

AU - De Vos, Winnok H.

AU - Baatout, Sarah

N1 - Score = 10

PY - 2014/8

Y1 - 2014/8

N2 - Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose (55 mSv) of ionizing radiation (IR), comprising a mixture of high linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibro¬blasts by inducing oxidative stress responsive genes. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.

AB - Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose (55 mSv) of ionizing radiation (IR), comprising a mixture of high linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibro¬blasts by inducing oxidative stress responsive genes. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.

KW - simulated space conditions

KW - microarrays

KW - cytoskeleton

KW - oxidative stress

KW - DNA damage

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_136881

UR - http://knowledgecentre.sckcen.be/so2/bibref/11807

U2 - 10.3892/ijmm.2014.1785

DO - 10.3892/ijmm.2014.1785

M3 - Article

VL - 34

SP - 606

EP - 615

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 2

ER -

ID: 70089