Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study

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Authors

  • Ellina Macaeva
  • Roel Quintens
  • Grainne Manning
  • Matthaeus Majewski
  • Ralf Kriehuber
  • Kamil Brzoska
  • Michael Abend
  • Sven Doucha-Senf
  • Dominik Oskamp
  • Sonja Strunz
  • Matthias Port
  • Christophe Badie

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Abstract

Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p0.5 Gy) measurements (p�.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.

Details

Original languageEnglish
Pages (from-to)87-98
JournalInternational Journal of Radiation Biology
Volume93
Issue number1
DOIs
Publication statusPublished - 14 Sep 2016

Keywords

  • biological dosimetry, biomarkers, dose-response, gene expression, ionising radiation, peripheral blood

ID: 1915870