Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study

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Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches : Second RENEB gene expression study. / Macaeva, Ellina; Quintens, Roel; Manning, Grainne; Majewski, Matthaeus; Kriehuber, Ralf; Brzoska, Kamil; Abend, Michael; Doucha-Senf, Sven; Oskamp, Dominik; Strunz, Sonja; Port, Matthias; Badie, Christophe; Quintens, Roel (Peer reviewer); Baatout, Sarah (Peer reviewer).

In: International Journal of Radiation Biology, Vol. 93, No. 1, 14.09.2016, p. 87-98.

Research output: Contribution to journalArticlepeer-review

Harvard

Macaeva, E, Quintens, R, Manning, G, Majewski, M, Kriehuber, R, Brzoska, K, Abend, M, Doucha-Senf, S, Oskamp, D, Strunz, S, Port, M, Badie, C, Quintens, R & Baatout, S 2016, 'Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study', International Journal of Radiation Biology, vol. 93, no. 1, pp. 87-98. https://doi.org/10.1080/09553002.2016.1227105

APA

Macaeva, E., Quintens, R., Manning, G., Majewski, M., Kriehuber, R., Brzoska, K., Abend, M., Doucha-Senf, S., Oskamp, D., Strunz, S., Port, M., Badie, C., Quintens, R., & Baatout, S. (2016). Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study. International Journal of Radiation Biology, 93(1), 87-98. https://doi.org/10.1080/09553002.2016.1227105

Author

Macaeva, Ellina ; Quintens, Roel ; Manning, Grainne ; Majewski, Matthaeus ; Kriehuber, Ralf ; Brzoska, Kamil ; Abend, Michael ; Doucha-Senf, Sven ; Oskamp, Dominik ; Strunz, Sonja ; Port, Matthias ; Badie, Christophe ; Quintens, Roel ; Baatout, Sarah. / Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches : Second RENEB gene expression study. In: International Journal of Radiation Biology. 2016 ; Vol. 93, No. 1. pp. 87-98.

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@article{6417c3c26b884481bdb5441f71ffe950,
title = "Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study",
abstract = "Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p0.5 Gy) measurements (p�.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.",
keywords = "biological dosimetry, biomarkers, dose-response, gene expression, ionising radiation, peripheral blood",
author = "Ellina Macaeva and Roel Quintens and Grainne Manning and Matthaeus Majewski and Ralf Kriehuber and Kamil Brzoska and Michael Abend and Sven Doucha-Senf and Dominik Oskamp and Sonja Strunz and Matthias Port and Christophe Badie and Roel Quintens and Sarah Baatout",
note = "score=10",
year = "2016",
month = sep,
day = "14",
doi = "10.1080/09553002.2016.1227105",
language = "English",
volume = "93",
pages = "87--98",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Taylor & Francis (CRC)",
number = "1",

}

RIS - Download

TY - JOUR

T1 - Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches

T2 - Second RENEB gene expression study

AU - Macaeva, Ellina

AU - Quintens, Roel

AU - Manning, Grainne

AU - Majewski, Matthaeus

AU - Kriehuber, Ralf

AU - Brzoska, Kamil

AU - Abend, Michael

AU - Doucha-Senf, Sven

AU - Oskamp, Dominik

AU - Strunz, Sonja

AU - Port, Matthias

AU - Badie, Christophe

A2 - Quintens, Roel

A2 - Baatout, Sarah

N1 - score=10

PY - 2016/9/14

Y1 - 2016/9/14

N2 - Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p0.5 Gy) measurements (p�.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.

AB - Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p0.5 Gy) measurements (p�.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.

KW - biological dosimetry

KW - biomarkers

KW - dose-response

KW - gene expression

KW - ionising radiation

KW - peripheral blood

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/20890152

U2 - 10.1080/09553002.2016.1227105

DO - 10.1080/09553002.2016.1227105

M3 - Article

VL - 93

SP - 87

EP - 98

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 1

ER -

ID: 1915870