Comparison of Antibiotic Resistance, Biofilm Formation and Conjugative Transfer of Staphylococcus and Enterococcus Isolates from International Space Station and Antarctic Research Station Concordia

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Comparison of Antibiotic Resistance, Biofilm Formation and Conjugative Transfer of Staphylococcus and Enterococcus Isolates from International Space Station and Antarctic Research Station Concordia. / Katarzyna, Schiwon; Arends, Karsten; Rogowski, Katja Marie; Fürch, Svea; Prescha, Katrin; Sakinc, Türkan; Van Houdt, Rob; Werner, Guido; Grohmann, Elisabeth.

In: Microbial Ecology, Vol. 65, No. 3, 04.2013, p. 638-651.

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Katarzyna, Schiwon ; Arends, Karsten ; Rogowski, Katja Marie ; Fürch, Svea ; Prescha, Katrin ; Sakinc, Türkan ; Van Houdt, Rob ; Werner, Guido ; Grohmann, Elisabeth. / Comparison of Antibiotic Resistance, Biofilm Formation and Conjugative Transfer of Staphylococcus and Enterococcus Isolates from International Space Station and Antarctic Research Station Concordia. In: Microbial Ecology. 2013 ; Vol. 65, No. 3. pp. 638-651.

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@article{23d35f498dda4057b0a7347823535c20,
title = "Comparison of Antibiotic Resistance, Biofilm Formation and Conjugative Transfer of Staphylococcus and Enterococcus Isolates from International Space Station and Antarctic Research Station Concordia",
abstract = "The International Space Station and the Antarctic Research Station Concordia are confined habitats in extreme environments. The human and habitat microflora can alter due to the special environmental conditions resulting in contamination and health risk for the crew. In this study, 29 isolates from the ISS and 55 from Concordia belonging to the genera Staphylococcus and Enterococcus were investigated. Resistance to one or more antibiotics was detected in 75.8% of the ISS and in 43.6% of the Concordia strains. The corresponding resistance genes were identified by polymerase chain reaction in 86% of the resistant ISS strains and in 18.2% of the resistant Concordia strains. Plasmids are present in 86.2% of the ISS and in 78.2% of the Concordia strains. Relaxase and/or transfer genes encoded on plasmids from gram-positive bacteria like pIP501, pRE25, pSK41, pGO1 and pT181 were detected in 86.2% of the ISS and in 52.7% of the Concordia strains. Most pSK41-homologous transfer genes were detected in ISS isolates belonging to coagulase-negative staphylococci. Biofilm formation was observed in 83% of the ISS and in 92.7% of the Concordia strains. In conclusion, ISS isolates were shown to encode more resistance genes and possess a higher gene transfer capacity than Concordia isolates.",
keywords = "Concordia, Antarctica, Contamination, Plasmid",
author = "Schiwon Katarzyna and Karsten Arends and Rogowski, {Katja Marie} and Svea F{\"u}rch and Katrin Prescha and T{\"u}rkan Sakinc and {Van Houdt}, Rob and Guido Werner and Elisabeth Grohmann",
note = "Score = 10",
year = "2013",
month = apr,
doi = "10.1007/s00248-013-0193-4",
language = "English",
volume = "65",
pages = "638--651",
journal = "Microbial Ecology",
issn = "0095-3628",
publisher = "Springer",
number = "3",

}

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TY - JOUR

T1 - Comparison of Antibiotic Resistance, Biofilm Formation and Conjugative Transfer of Staphylococcus and Enterococcus Isolates from International Space Station and Antarctic Research Station Concordia

AU - Katarzyna, Schiwon

AU - Arends, Karsten

AU - Rogowski, Katja Marie

AU - Fürch, Svea

AU - Prescha, Katrin

AU - Sakinc, Türkan

AU - Van Houdt, Rob

AU - Werner, Guido

AU - Grohmann, Elisabeth

N1 - Score = 10

PY - 2013/4

Y1 - 2013/4

N2 - The International Space Station and the Antarctic Research Station Concordia are confined habitats in extreme environments. The human and habitat microflora can alter due to the special environmental conditions resulting in contamination and health risk for the crew. In this study, 29 isolates from the ISS and 55 from Concordia belonging to the genera Staphylococcus and Enterococcus were investigated. Resistance to one or more antibiotics was detected in 75.8% of the ISS and in 43.6% of the Concordia strains. The corresponding resistance genes were identified by polymerase chain reaction in 86% of the resistant ISS strains and in 18.2% of the resistant Concordia strains. Plasmids are present in 86.2% of the ISS and in 78.2% of the Concordia strains. Relaxase and/or transfer genes encoded on plasmids from gram-positive bacteria like pIP501, pRE25, pSK41, pGO1 and pT181 were detected in 86.2% of the ISS and in 52.7% of the Concordia strains. Most pSK41-homologous transfer genes were detected in ISS isolates belonging to coagulase-negative staphylococci. Biofilm formation was observed in 83% of the ISS and in 92.7% of the Concordia strains. In conclusion, ISS isolates were shown to encode more resistance genes and possess a higher gene transfer capacity than Concordia isolates.

AB - The International Space Station and the Antarctic Research Station Concordia are confined habitats in extreme environments. The human and habitat microflora can alter due to the special environmental conditions resulting in contamination and health risk for the crew. In this study, 29 isolates from the ISS and 55 from Concordia belonging to the genera Staphylococcus and Enterococcus were investigated. Resistance to one or more antibiotics was detected in 75.8% of the ISS and in 43.6% of the Concordia strains. The corresponding resistance genes were identified by polymerase chain reaction in 86% of the resistant ISS strains and in 18.2% of the resistant Concordia strains. Plasmids are present in 86.2% of the ISS and in 78.2% of the Concordia strains. Relaxase and/or transfer genes encoded on plasmids from gram-positive bacteria like pIP501, pRE25, pSK41, pGO1 and pT181 were detected in 86.2% of the ISS and in 52.7% of the Concordia strains. Most pSK41-homologous transfer genes were detected in ISS isolates belonging to coagulase-negative staphylococci. Biofilm formation was observed in 83% of the ISS and in 92.7% of the Concordia strains. In conclusion, ISS isolates were shown to encode more resistance genes and possess a higher gene transfer capacity than Concordia isolates.

KW - Concordia

KW - Antarctica

KW - Contamination

KW - Plasmid

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_127406

UR - http://knowledgecentre.sckcen.be/so2/bibref/10143

U2 - 10.1007/s00248-013-0193-4

DO - 10.1007/s00248-013-0193-4

M3 - Article

VL - 65

SP - 638

EP - 651

JO - Microbial Ecology

JF - Microbial Ecology

SN - 0095-3628

IS - 3

ER -

ID: 117090