Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle

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Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle. / De Vos, Winnok; Hoebe, R.A.; Joss, G.H.; Haffmans, W; Baatout, Sarah; Van Oostveldt, Patrick; Manders, E.M.; Jacquet, Paul (Peer reviewer).

In: Cytometry part A, Vol. 75A, No. 5, 18.12.2009, p. 428-439.

Research output: Contribution to journalArticlepeer-review

Harvard

De Vos, W, Hoebe, RA, Joss, GH, Haffmans, W, Baatout, S, Van Oostveldt, P, Manders, EM & Jacquet, P 2009, 'Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle', Cytometry part A, vol. 75A, no. 5, pp. 428-439. https://doi.org/10.1002/cyto.a.20699

APA

De Vos, W., Hoebe, R. A., Joss, G. H., Haffmans, W., Baatout, S., Van Oostveldt, P., Manders, E. M., & Jacquet, P. (2009). Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle. Cytometry part A, 75A(5), 428-439. https://doi.org/10.1002/cyto.a.20699

Vancouver

De Vos W, Hoebe RA, Joss GH, Haffmans W, Baatout S, Van Oostveldt P et al. Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle. Cytometry part A. 2009 Dec 18;75A(5):428-439. https://doi.org/10.1002/cyto.a.20699

Author

De Vos, Winnok ; Hoebe, R.A. ; Joss, G.H. ; Haffmans, W ; Baatout, Sarah ; Van Oostveldt, Patrick ; Manders, E.M. ; Jacquet, Paul. / Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle. In: Cytometry part A. 2009 ; Vol. 75A, No. 5. pp. 428-439.

Bibtex - Download

@article{99298f1577724c31a16ccffbec911928,
title = "Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle",
abstract = "Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics.",
keywords = "cell, cell cycle, telomere, microscopy",
author = "{De Vos}, Winnok and R.A. Hoebe and G.H. Joss and W Haffmans and Sarah Baatout and {Van Oostveldt}, Patrick and E.M. Manders and Paul Jacquet",
note = "Score = 10",
year = "2009",
month = dec,
day = "18",
doi = "10.1002/cyto.a.20699",
language = "English",
volume = "75A",
pages = "428--439",
journal = "Cytometry part A",
issn = "1552-4922",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "5",

}

RIS - Download

TY - JOUR

T1 - Controlled Light Exposure Microscopy Reveals Dynamic Telomere Microterritories Throughout the Cell Cycle

AU - De Vos, Winnok

AU - Hoebe, R.A.

AU - Joss, G.H.

AU - Haffmans, W

AU - Baatout, Sarah

AU - Van Oostveldt, Patrick

AU - Manders, E.M.

A2 - Jacquet, Paul

N1 - Score = 10

PY - 2009/12/18

Y1 - 2009/12/18

N2 - Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics.

AB - Telomeres are complex end structures that confer functional integrity and positional stability to human chromosomes. Despite their critical importance, there is no clear view on telomere organization in cycling human cells and their dynamic behavior throughout the cell cycle. We investigated spatiotemporal organization of telomeres in living human ECV-304 cells stably expressing telomere binding proteins TRF1 and TRF2 fused to mCitrine using four dimensional microscopy. We thereby made use of controlled light exposure microscopy (CLEM), a novel technology that strongly reduces photodamage by limiting excitation in parts of the image where full exposure is not needed. We found that telomeres share small territories where they dynamically associate. These territories are preferentially positioned at the interface of chromatin domains. TRF1 and TRF2 are abundantly present in these territories but not firmly bound. At the onset of mitosis, the bulk of TRF protein dissociates from telomere regions, territories disintegrate and individual telomeres become faintly visible. The combination of stable cell lines, CLEM and cytometry proved essential in providing novel insights in compartment-based nuclear organization and may serve as a model approach for investigating telomere-driven genome-instability and studying long-term nuclear dynamics.

KW - cell

KW - cell cycle

KW - telomere

KW - microscopy

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_104802

UR - http://knowledgecentre.sckcen.be/so2/bibref/6819

U2 - 10.1002/cyto.a.20699

DO - 10.1002/cyto.a.20699

M3 - Article

VL - 75A

SP - 428

EP - 439

JO - Cytometry part A

JF - Cytometry part A

SN - 1552-4922

IS - 5

ER -

ID: 227802