Copper resistance mediates long-term survival of Cupriavidus metallidurans in wet contact with metallic copper

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Copper resistance mediates long-term survival of Cupriavidus metallidurans in wet contact with metallic copper. / Maertens, Laurens; Coninx, Ilse; Claesen, Jürgen; Leys, Natalie; Matroule, Jean-Yves; Van Houdt, Rob.

In: Frontiers in Microbiology, 03.06.2020, p. 1-11.

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@article{8ceae2bdebdf4f348208d396adf6df78,
title = "Copper resistance mediates long-term survival of Cupriavidus metallidurans in wet contact with metallic copper",
abstract = "Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.",
keywords = "Copper, Cupriavidus, Drinking water, VBNC, Heavy metal resistance",
author = "Laurens Maertens and Ilse Coninx and J{\"u}rgen Claesen and Natalie Leys and Jean-Yves Matroule and {Van Houdt}, Rob",
note = "Score=10",
year = "2020",
month = "6",
day = "3",
doi = "10.3389/fmicb.2020.01208",
language = "English",
pages = "1--11",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers",

}

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TY - JOUR

T1 - Copper resistance mediates long-term survival of Cupriavidus metallidurans in wet contact with metallic copper

AU - Maertens, Laurens

AU - Coninx, Ilse

AU - Claesen, Jürgen

AU - Leys, Natalie

AU - Matroule, Jean-Yves

AU - Van Houdt, Rob

N1 - Score=10

PY - 2020/6/3

Y1 - 2020/6/3

N2 - Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.

AB - Metallic copper to combat bacterial proliferation in drinking water systems is being investigated as an attractive alternative to existing strategies. A potential obstacle to this approach is the induction of metal resistance mechanisms in contaminating bacteria, that could severely impact inactivation efficacy. Thus far, the role of these resistance mechanisms has not been studied in conditions relevant to drinking water systems. Therefore, we evaluated the inactivation kinetics of Cupriavidus metallidurans CH34 in contact with metallic copper in drinking water. Viability and membrane permeability were examined for 9 days through viable counts and flow cytometry. After an initial drop in viable count, a significant recovery was observed starting after 48 h. This behavior could be explained by either a recovery from an injured/viable-but-non-culturable state or regrowth of surviving cells metabolizing lysed cells. Either hypothesis would necessitate an induction of copper resistance mechanisms, since no recovery was seen in a CH34 mutant strain lacking metal resistance mechanisms, while being more pronounced when copper resistance mechanisms were pre-induced. Interestingly, no biofilms were formed on the copper surface, while extensive biofilm formation was observed on the stainless steel control plates. When CH34 cells in water were supplied with CuSO4, a similar initial decrease in viable counts was observed, but cells recovered fully after 7 days. In conclusion, we have shown that long-term bacterial survival in the presence of a copper surface is possible upon the induction of metal resistance mechanisms. This observation may have important consequences in the context of the increasing use of copper as an antimicrobial surface, especially in light of potential co-selection for metal and antimicrobial resistance.

KW - Copper

KW - Cupriavidus

KW - Drinking water

KW - VBNC

KW - Heavy metal resistance

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/open/41514547

U2 - 10.3389/fmicb.2020.01208

DO - 10.3389/fmicb.2020.01208

M3 - Article

SP - 1

EP - 11

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -

ID: 6978825