Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system

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@article{05f7277be2e94a75b36930a0289cfde7,
title = "Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system",
abstract = "In vivo studies on X-irradiated mice have shown that structural chromosome aberrations can be induced in female germ cells and that the radiation-induced chromosomal damage strongly depends on the stage of maturation reached by the oocytes at the time of irradiation. In the present study, the sensitivity of oocytes to radiation-induction of chromosome damage was evaluated at 2 different stages, using a recently developed method of in vitro culture covering a crucial period of follicle/oocyte growth and maturation. A key feature of this system is that growth and development of all follicles is perfectly synchronized, due to the selection of a narrow class of follicles to start-off culture. This allows irradiation of well characterized and homogenous populations of follicles, in contrast to the situation prevailing in vivo. Follicles were X-irradiated with either 2 or 4 Gy, on day 0 of the culture (early preantral follicles with 1-2 cell layers) or on day 12, 3 h after hormonal stimulation of ovulation (antral Graafian follicles). Ovulated oocytes, blocked in MI by colchicine, were fixed 16 h after hormonal stimulation and analyzed for chromosome aberrations. The results confirmed the high radiosensitivity of oocytes 2 weeks prior to ovulation and the even higher radiosensitivity of those irradiated a few hours before ovulation, underlining the suitability of the in vitro system for further studies on the genetic effects of ionising radiation in female mammals.",
keywords = "MI oocytes, X-irradiation, chromosome aberrations, in vitro culture",
author = "Paul Jacquet and Iris Adriaens and Jasmine Buset and Mieke Neefs and Sarah Baatout and Max Mergeay",
note = "Score = 10",
year = "2005",
language = "English",
volume = "583",
pages = "168--177",
journal = "Mutation Research",
issn = "1383-5718",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system

AU - Jacquet, Paul

AU - Adriaens, Iris

AU - Buset, Jasmine

AU - Neefs, Mieke

A2 - Baatout, Sarah

A2 - Mergeay, Max

N1 - Score = 10

PY - 2005

Y1 - 2005

N2 - In vivo studies on X-irradiated mice have shown that structural chromosome aberrations can be induced in female germ cells and that the radiation-induced chromosomal damage strongly depends on the stage of maturation reached by the oocytes at the time of irradiation. In the present study, the sensitivity of oocytes to radiation-induction of chromosome damage was evaluated at 2 different stages, using a recently developed method of in vitro culture covering a crucial period of follicle/oocyte growth and maturation. A key feature of this system is that growth and development of all follicles is perfectly synchronized, due to the selection of a narrow class of follicles to start-off culture. This allows irradiation of well characterized and homogenous populations of follicles, in contrast to the situation prevailing in vivo. Follicles were X-irradiated with either 2 or 4 Gy, on day 0 of the culture (early preantral follicles with 1-2 cell layers) or on day 12, 3 h after hormonal stimulation of ovulation (antral Graafian follicles). Ovulated oocytes, blocked in MI by colchicine, were fixed 16 h after hormonal stimulation and analyzed for chromosome aberrations. The results confirmed the high radiosensitivity of oocytes 2 weeks prior to ovulation and the even higher radiosensitivity of those irradiated a few hours before ovulation, underlining the suitability of the in vitro system for further studies on the genetic effects of ionising radiation in female mammals.

AB - In vivo studies on X-irradiated mice have shown that structural chromosome aberrations can be induced in female germ cells and that the radiation-induced chromosomal damage strongly depends on the stage of maturation reached by the oocytes at the time of irradiation. In the present study, the sensitivity of oocytes to radiation-induction of chromosome damage was evaluated at 2 different stages, using a recently developed method of in vitro culture covering a crucial period of follicle/oocyte growth and maturation. A key feature of this system is that growth and development of all follicles is perfectly synchronized, due to the selection of a narrow class of follicles to start-off culture. This allows irradiation of well characterized and homogenous populations of follicles, in contrast to the situation prevailing in vivo. Follicles were X-irradiated with either 2 or 4 Gy, on day 0 of the culture (early preantral follicles with 1-2 cell layers) or on day 12, 3 h after hormonal stimulation of ovulation (antral Graafian follicles). Ovulated oocytes, blocked in MI by colchicine, were fixed 16 h after hormonal stimulation and analyzed for chromosome aberrations. The results confirmed the high radiosensitivity of oocytes 2 weeks prior to ovulation and the even higher radiosensitivity of those irradiated a few hours before ovulation, underlining the suitability of the in vitro system for further studies on the genetic effects of ionising radiation in female mammals.

KW - MI oocytes

KW - X-irradiation

KW - chromosome aberrations

KW - in vitro culture

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_27305

UR - http://knowledgecentre.sckcen.be/so2/bibref/3276

M3 - Article

VL - 583

SP - 168

EP - 177

JO - Mutation Research

JF - Mutation Research

SN - 1383-5718

ER -

ID: 268775