Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts

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Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts. / Potters, Geert; Jansen, Marcel A.K.; Horemans, Nele; Guisez, Yves; Pasternak, Taras; Vandenhove, Hildegarde (Peer reviewer); Vanmarcke, Hans (Peer reviewer).

In: In Vitro Cellular & Developmental Biology, Vol. 46, No. 3, 29.01.2010, p. 289-296.

Research output: Contribution to journalArticlepeer-review

Harvard

Potters, G, Jansen, MAK, Horemans, N, Guisez, Y, Pasternak, T, Vandenhove, H & Vanmarcke, H 2010, 'Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts', In Vitro Cellular & Developmental Biology, vol. 46, no. 3, pp. 289-296. https://doi.org/10.1007/s11627-009-9266-y

APA

Vancouver

Potters G, Jansen MAK, Horemans N, Guisez Y, Pasternak T, Vandenhove H et al. Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts. In Vitro Cellular & Developmental Biology. 2010 Jan 29;46(3):289-296. https://doi.org/10.1007/s11627-009-9266-y

Author

Potters, Geert ; Jansen, Marcel A.K. ; Horemans, Nele ; Guisez, Yves ; Pasternak, Taras ; Vandenhove, Hildegarde ; Vanmarcke, Hans. / Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts. In: In Vitro Cellular & Developmental Biology. 2010 ; Vol. 46, No. 3. pp. 289-296.

Bibtex - Download

@article{1177ed5c8b8a49a4b0e17402b72436e3,
title = "Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts",
abstract = "A Nicotiana tabacum L. SR-1 leaf protoplast system was used to study the effects of dehydroascorbate and glutathione on cellular development. We found that dehydroascorbate is readily taken up by protoplasts and internally reduced to ascorbate. Concomitantly, cell division was inhibited and cell expansion stimulated. In this respect, dehydroascorbate counteracted auxin-mediated leaf protoplast development. In contrast to the effects of dehydroascorbate, glutathione-induced cell dedifferentiation, and this effect is similar to that mediated by high auxin concentrations. We conclude that dehydroascorbate and glutathione affect the auxin-mediated regulation of cellular development. Therefore, the biological role of these compounds extends beyond stress tolerance and defense.",
keywords = "dedifferentiation, dehydroascorbate, glutathione, Nicotiana tabacum, stress response",
author = "Geert Potters and Jansen, {Marcel A.K.} and Nele Horemans and Yves Guisez and Taras Pasternak and Hildegarde Vandenhove and Hans Vanmarcke",
note = "Score = 10",
year = "2010",
month = jan,
day = "29",
doi = "10.1007/s11627-009-9266-y",
language = "English",
volume = "46",
pages = "289--296",
journal = "In Vitro Cellular & Developmental Biology",
issn = "1054-5476",
publisher = "SIVB - Society for In Vitro Biology",
number = "3",

}

RIS - Download

TY - JOUR

T1 - Dehydroascorbate and glutathione regulate the cellular development of Nicotiana tabacum L. SR-1 protoplasts

AU - Potters, Geert

AU - Jansen, Marcel A.K.

AU - Horemans, Nele

AU - Guisez, Yves

AU - Pasternak, Taras

A2 - Vandenhove, Hildegarde

A2 - Vanmarcke, Hans

N1 - Score = 10

PY - 2010/1/29

Y1 - 2010/1/29

N2 - A Nicotiana tabacum L. SR-1 leaf protoplast system was used to study the effects of dehydroascorbate and glutathione on cellular development. We found that dehydroascorbate is readily taken up by protoplasts and internally reduced to ascorbate. Concomitantly, cell division was inhibited and cell expansion stimulated. In this respect, dehydroascorbate counteracted auxin-mediated leaf protoplast development. In contrast to the effects of dehydroascorbate, glutathione-induced cell dedifferentiation, and this effect is similar to that mediated by high auxin concentrations. We conclude that dehydroascorbate and glutathione affect the auxin-mediated regulation of cellular development. Therefore, the biological role of these compounds extends beyond stress tolerance and defense.

AB - A Nicotiana tabacum L. SR-1 leaf protoplast system was used to study the effects of dehydroascorbate and glutathione on cellular development. We found that dehydroascorbate is readily taken up by protoplasts and internally reduced to ascorbate. Concomitantly, cell division was inhibited and cell expansion stimulated. In this respect, dehydroascorbate counteracted auxin-mediated leaf protoplast development. In contrast to the effects of dehydroascorbate, glutathione-induced cell dedifferentiation, and this effect is similar to that mediated by high auxin concentrations. We conclude that dehydroascorbate and glutathione affect the auxin-mediated regulation of cellular development. Therefore, the biological role of these compounds extends beyond stress tolerance and defense.

KW - dedifferentiation

KW - dehydroascorbate

KW - glutathione

KW - Nicotiana tabacum

KW - stress response

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_107546

UR - http://knowledgecentre.sckcen.be/so2/bibref/7192

U2 - 10.1007/s11627-009-9266-y

DO - 10.1007/s11627-009-9266-y

M3 - Article

VL - 46

SP - 289

EP - 296

JO - In Vitro Cellular & Developmental Biology

JF - In Vitro Cellular & Developmental Biology

SN - 1054-5476

IS - 3

ER -

ID: 247840