Deuteros 2.0: peptide-level significance testing of data from hydrogen deuterium exchange mass spectrometry

Research output: Contribution to journalArticlepeer-review

Authors

  • Andy M. Lau
  • Jürgen Claesen
  • Kjetil Hansen
  • Argyris Politis

Institutes & Expert groups

  • King's College London
  • Uhasselt - Hasselt University

Documents & links

Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming increasing routine for monitoring changes in the structural dynamics of proteins. Differential HDX-MS allows comparison of protein states, such as in the absence or presence of a ligand. This can be used to attribute changes in conformation to binding events, allowing the mapping of entire conformational networks. As such, the number of necessary cross-state comparisons quickly increases as additional states are introduced to the system of study. There are currently very few software packages available that offer quick and informative comparison of HDX-MS datasets and even fewer which offer statistical analysis and advanced visualization. Following the feedback from our original software Deuteros, we present Deuteros 2.0 which has been redesigned from the ground up to fulfill a greater role in the HDX-MS analysis pipeline. Deuteros 2.0 features a repertoire of facilities for back exchange correction, data summarization, peptidelevel statistical analysis and advanced data plotting features.

Details

Original languageEnglish
Pages (from-to)270-272
Number of pages3
JournalBioinformatics
Volume37
Issue number2
DOIs
Publication statusPublished - 8 Jan 2021

Keywords

  • HDX-MS, Hydrogen deuterium exchange mass spectrometry, Deuteros 2.0

ID: 7498116