Different Responsiveness of Endothelial Cells to Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Added to Culture Media Under Gravity and Simulated Microgravity

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Different Responsiveness of Endothelial Cells to Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Added to Culture Media Under Gravity and Simulated Microgravity. / Grimm, Daniela; Bauer, Johann; Ulbrich, Claudia; Westphal, Krist; Wehland, Markus; Infanger, Manfred; Aleshcheva, Ganna; Pietsch, Jessica; Ghardi, Myriam; Beck, Michaël; El Saghire, Houssein; de Saint-Georges, Louis; Baatout, Sarah.

In: Tissue Engineering Part A, Vol. 16, No. 5, 04.05.2010, p. 1559-1573.

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Grimm, Daniela ; Bauer, Johann ; Ulbrich, Claudia ; Westphal, Krist ; Wehland, Markus ; Infanger, Manfred ; Aleshcheva, Ganna ; Pietsch, Jessica ; Ghardi, Myriam ; Beck, Michaël ; El Saghire, Houssein ; de Saint-Georges, Louis ; Baatout, Sarah. / Different Responsiveness of Endothelial Cells to Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Added to Culture Media Under Gravity and Simulated Microgravity. In: Tissue Engineering Part A. 2010 ; Vol. 16, No. 5. pp. 1559-1573.

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@article{62012ad591fa4ea0a9782f86c911b7d4,
title = "Different Responsiveness of Endothelial Cells to Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Added to Culture Media Under Gravity and Simulated Microgravity",
abstract = "When incubated under simulated microgravity (s-mg), endothelial cells (EC) form tubular structures that resemble vascular intimas. This delayed formation of 3D EC structures begins between the 5th and 7th day of culturing EC under conditions of s-mg. With the aim of learning about this initial phase of tubular structure formation, NFkBp65 protein content was similar in all cell populations, but gene and protein expression of phosphokinase A catalytic subunit, phosphokinase Ca, and extracellular signal-regulated kinases 1 and 2 was altered in cells cultured under s-mg. In contrast to controls, the 7-day-old s-mg cultures contained 3D aggregates with proliferating cells, enhanced numbers of necrotic cells, and osteopontin-negative EC as well as supernatants with reduced quantities of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), soluble TNFRSF5, TNFSF5, intercellular adhesion molecule-1, tumor necrosis factor receptor 2, IL-18, complement C3, and von Willebrand factor. VEGF and=or bFGF (10 ng=mL) application influenced the accumulation of proteins in supernatants more profoundly under 1 g than under s-mg. These findings provide evidence that phosphokinase Ca plays a key role in tube formation. Improving the interaction of VEGF and=or bFGF with EC under s-mg could enhance the engineering of vascular intimas.",
keywords = "Endothelial cells, microgravity, simulated microgravity, VEGF, bFGF, TNFR",
author = "Daniela Grimm and Johann Bauer and Claudia Ulbrich and Krist Westphal and Markus Wehland and Manfred Infanger and Ganna Aleshcheva and Jessica Pietsch and Myriam Ghardi and Micha{\"e}l Beck and {El Saghire}, Houssein and {de Saint-Georges}, Louis and Sarah Baatout",
note = "Score = 10",
year = "2010",
month = "5",
day = "4",
doi = "10.1089/ten.tea.2009.0524",
language = "English",
volume = "16",
pages = "1559--1573",
journal = "Tissue Engineering Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

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TY - JOUR

T1 - Different Responsiveness of Endothelial Cells to Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Added to Culture Media Under Gravity and Simulated Microgravity

AU - Grimm, Daniela

AU - Bauer, Johann

AU - Ulbrich, Claudia

AU - Westphal, Krist

AU - Wehland, Markus

AU - Infanger, Manfred

AU - Aleshcheva, Ganna

AU - Pietsch, Jessica

AU - Ghardi, Myriam

AU - Beck, Michaël

AU - El Saghire, Houssein

AU - de Saint-Georges, Louis

AU - Baatout, Sarah

N1 - Score = 10

PY - 2010/5/4

Y1 - 2010/5/4

N2 - When incubated under simulated microgravity (s-mg), endothelial cells (EC) form tubular structures that resemble vascular intimas. This delayed formation of 3D EC structures begins between the 5th and 7th day of culturing EC under conditions of s-mg. With the aim of learning about this initial phase of tubular structure formation, NFkBp65 protein content was similar in all cell populations, but gene and protein expression of phosphokinase A catalytic subunit, phosphokinase Ca, and extracellular signal-regulated kinases 1 and 2 was altered in cells cultured under s-mg. In contrast to controls, the 7-day-old s-mg cultures contained 3D aggregates with proliferating cells, enhanced numbers of necrotic cells, and osteopontin-negative EC as well as supernatants with reduced quantities of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), soluble TNFRSF5, TNFSF5, intercellular adhesion molecule-1, tumor necrosis factor receptor 2, IL-18, complement C3, and von Willebrand factor. VEGF and=or bFGF (10 ng=mL) application influenced the accumulation of proteins in supernatants more profoundly under 1 g than under s-mg. These findings provide evidence that phosphokinase Ca plays a key role in tube formation. Improving the interaction of VEGF and=or bFGF with EC under s-mg could enhance the engineering of vascular intimas.

AB - When incubated under simulated microgravity (s-mg), endothelial cells (EC) form tubular structures that resemble vascular intimas. This delayed formation of 3D EC structures begins between the 5th and 7th day of culturing EC under conditions of s-mg. With the aim of learning about this initial phase of tubular structure formation, NFkBp65 protein content was similar in all cell populations, but gene and protein expression of phosphokinase A catalytic subunit, phosphokinase Ca, and extracellular signal-regulated kinases 1 and 2 was altered in cells cultured under s-mg. In contrast to controls, the 7-day-old s-mg cultures contained 3D aggregates with proliferating cells, enhanced numbers of necrotic cells, and osteopontin-negative EC as well as supernatants with reduced quantities of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), soluble TNFRSF5, TNFSF5, intercellular adhesion molecule-1, tumor necrosis factor receptor 2, IL-18, complement C3, and von Willebrand factor. VEGF and=or bFGF (10 ng=mL) application influenced the accumulation of proteins in supernatants more profoundly under 1 g than under s-mg. These findings provide evidence that phosphokinase Ca plays a key role in tube formation. Improving the interaction of VEGF and=or bFGF with EC under s-mg could enhance the engineering of vascular intimas.

KW - Endothelial cells

KW - microgravity

KW - simulated microgravity

KW - VEGF

KW - bFGF

KW - TNFR

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_105800

UR - http://knowledgecentre.sckcen.be/so2/bibref/6978

U2 - 10.1089/ten.tea.2009.0524

DO - 10.1089/ten.tea.2009.0524

M3 - Article

VL - 16

SP - 1559

EP - 1573

JO - Tissue Engineering Part A

JF - Tissue Engineering Part A

SN - 1937-3341

IS - 5

ER -

ID: 355099