Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi in an autotrophic nitrifying biofilm reactor as depicted by molecular analyses and mathematical modelling

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Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi in an autotrophic nitrifying biofilm reactor as depicted by molecular analyses and mathematical modelling. / Montras, Anna; Pycke, Benny; Boon, Nico; Godia, Francesc; Mergeay, Max; Hendrickx, Larissa; Pérez, Julio.

In: Water Research, Vol. 42, No. 6-7, 03.2008, p. 1700-1714.

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@article{118412c99c4c4a8b91795a60c599da6a,
title = "Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi in an autotrophic nitrifying biofilm reactor as depicted by molecular analyses and mathematical modelling",
abstract = "The autotrophic two-species biofilm from the packed bed reactor of a life-support system, containing Nitrosomonas europaea ATCC 19718 and Nitrobacter winogradskyi ATCC 25391, was analysed after 4.8 years of continuous operation performing complete nitrification. Realtime quantitative polymerase chain reaction (Q-PCR) was used to quantify N. europaea and N. winogradskyi along the vertical axis of the reactor, revealing a spatial segregation of N. europaea and N. winogradskyi. The main parameters influencing the spatial segregation of both nitrifiers along the bed were assessed through a multi-species one-dimensional biofilm model generated with AQUASIM software. The factor that contributed the most to this distribution profile was a small deviation from the flow pattern of a perfectly mixed tank towards plug-flow. The results indicate that the model can estimate the impact of specific biofilm parameters and predict the nitrification efficiency and population dynamics of a multispecies biofilm",
keywords = "Ammonium oxidation, AQUASIM, FISH, Juxta-positioning, Model, Nitrite oxidation, Real-time PCR",
author = "Anna Montras and Benny Pycke and Nico Boon and Francesc Godia and Max Mergeay and Larissa Hendrickx and Julio P{\'e}rez",
note = "Score = 10",
year = "2008",
month = mar,
doi = "10.1016/j.watres.2007.10.025",
language = "English",
volume = "42",
pages = "1700--1714",
journal = "Water Research",
issn = "0043-1354",
publisher = "IWA Publishing",
number = "6-7",

}

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TY - JOUR

T1 - Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi in an autotrophic nitrifying biofilm reactor as depicted by molecular analyses and mathematical modelling

AU - Montras, Anna

AU - Pycke, Benny

AU - Boon, Nico

AU - Godia, Francesc

AU - Mergeay, Max

AU - Hendrickx, Larissa

AU - Pérez, Julio

N1 - Score = 10

PY - 2008/3

Y1 - 2008/3

N2 - The autotrophic two-species biofilm from the packed bed reactor of a life-support system, containing Nitrosomonas europaea ATCC 19718 and Nitrobacter winogradskyi ATCC 25391, was analysed after 4.8 years of continuous operation performing complete nitrification. Realtime quantitative polymerase chain reaction (Q-PCR) was used to quantify N. europaea and N. winogradskyi along the vertical axis of the reactor, revealing a spatial segregation of N. europaea and N. winogradskyi. The main parameters influencing the spatial segregation of both nitrifiers along the bed were assessed through a multi-species one-dimensional biofilm model generated with AQUASIM software. The factor that contributed the most to this distribution profile was a small deviation from the flow pattern of a perfectly mixed tank towards plug-flow. The results indicate that the model can estimate the impact of specific biofilm parameters and predict the nitrification efficiency and population dynamics of a multispecies biofilm

AB - The autotrophic two-species biofilm from the packed bed reactor of a life-support system, containing Nitrosomonas europaea ATCC 19718 and Nitrobacter winogradskyi ATCC 25391, was analysed after 4.8 years of continuous operation performing complete nitrification. Realtime quantitative polymerase chain reaction (Q-PCR) was used to quantify N. europaea and N. winogradskyi along the vertical axis of the reactor, revealing a spatial segregation of N. europaea and N. winogradskyi. The main parameters influencing the spatial segregation of both nitrifiers along the bed were assessed through a multi-species one-dimensional biofilm model generated with AQUASIM software. The factor that contributed the most to this distribution profile was a small deviation from the flow pattern of a perfectly mixed tank towards plug-flow. The results indicate that the model can estimate the impact of specific biofilm parameters and predict the nitrification efficiency and population dynamics of a multispecies biofilm

KW - Ammonium oxidation

KW - AQUASIM

KW - FISH

KW - Juxta-positioning

KW - Model

KW - Nitrite oxidation

KW - Real-time PCR

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_94023

UR - http://knowledgecentre.sckcen.be/so2/bibref/5472

U2 - 10.1016/j.watres.2007.10.025

DO - 10.1016/j.watres.2007.10.025

M3 - Article

VL - 42

SP - 1700

EP - 1714

JO - Water Research

JF - Water Research

SN - 0043-1354

IS - 6-7

ER -

ID: 155950