Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

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@article{b6ee5d84c09147f7a89c39f18f2fe34c,
title = "Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation",
abstract = "Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness. This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses of accelerated 13C-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines.",
keywords = "carbon ion irradiation, colony survival assay, motility genes, PC3 prostate adenocarcinoma, Caco-2 colon adenocarcinoma, gene expression",
author = "Annelies Suetens and Marjan Moreels and Roel Quintens and Els Soors and Jasmine Buset and {Chiriotti Alvarez}, Sabina and Kevin Tabury and Vincent GREGOIRE and Sarah Baatout",
note = "Score = 10",
year = "2015",
month = "1",
day = "15",
doi = "10.1093/jrr/rru070",
language = "English",
volume = "56",
pages = "11--21",
journal = "Journal of Radiation Research",
issn = "1349-9157",
publisher = "Oxford University Press",
number = "1",

}

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TY - JOUR

T1 - Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

AU - Suetens, Annelies

AU - Moreels, Marjan

AU - Quintens, Roel

AU - Soors, Els

AU - Buset, Jasmine

AU - Chiriotti Alvarez, Sabina

AU - Tabury, Kevin

AU - GREGOIRE, Vincent

AU - Baatout, Sarah

N1 - Score = 10

PY - 2015/1/15

Y1 - 2015/1/15

N2 - Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness. This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses of accelerated 13C-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines.

AB - Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness. This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses of accelerated 13C-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines.

KW - carbon ion irradiation

KW - colony survival assay

KW - motility genes

KW - PC3 prostate adenocarcinoma

KW - Caco-2 colon adenocarcinoma

KW - gene expression

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_138193

UR - http://knowledgecentre.sckcen.be/so2/bibref/12210

U2 - 10.1093/jrr/rru070

DO - 10.1093/jrr/rru070

M3 - Article

VL - 56

SP - 11

EP - 21

JO - Journal of Radiation Research

JF - Journal of Radiation Research

SN - 1349-9157

IS - 1

ER -

ID: 340343