Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung

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Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung. / Dingemans, Jozef; Monsieurs, Pieter; Yu, Sung-Huan; Crabbé, Aurélie; Förstner, Konrad U.; Malfroot, Anne; Cornelis, Pierre; Van Houdt, Rob.

In: mBio, Vol. 7, No. 4, e00813-16, 02.08.2016.

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Dingemans, Jozef ; Monsieurs, Pieter ; Yu, Sung-Huan ; Crabbé, Aurélie ; Förstner, Konrad U. ; Malfroot, Anne ; Cornelis, Pierre ; Van Houdt, Rob. / Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung. In: mBio. 2016 ; Vol. 7, No. 4.

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@article{84808128887a43d48d72509985a62332,
title = "Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung",
abstract = "Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.",
keywords = "Pseudomonas, cystic fibrosis, Rotating Wall Vessel",
author = "Jozef Dingemans and Pieter Monsieurs and Sung-Huan Yu and Aur{\'e}lie Crabb{\'e} and F{\"o}rstner, {Konrad U.} and Anne Malfroot and Pierre Cornelis and {Van Houdt}, Rob",
note = "Score=10",
year = "2016",
month = aug,
day = "2",
doi = "10.1128/mBio.00813-16",
language = "English",
volume = "7",
journal = "mBio",
issn = "2150-7511",
publisher = "ASMsociety - American Society for Microbiology",
number = "4",

}

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TY - JOUR

T1 - Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung

AU - Dingemans, Jozef

AU - Monsieurs, Pieter

AU - Yu, Sung-Huan

AU - Crabbé, Aurélie

AU - Förstner, Konrad U.

AU - Malfroot, Anne

AU - Cornelis, Pierre

AU - Van Houdt, Rob

N1 - Score=10

PY - 2016/8/2

Y1 - 2016/8/2

N2 - Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.

AB - Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.

KW - Pseudomonas

KW - cystic fibrosis

KW - Rotating Wall Vessel

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/18428989

U2 - 10.1128/mBio.00813-16

DO - 10.1128/mBio.00813-16

M3 - Article

VL - 7

JO - mBio

JF - mBio

SN - 2150-7511

IS - 4

M1 - e00813-16

ER -

ID: 1299997