Effect of X- and Gamma Rays on DNA in Human Cells

Research output: Contribution to journalArticlepeer-review

Standard

Effect of X- and Gamma Rays on DNA in Human Cells. / Faraj, Kharman A.; Elias, Mazin M.; Al-Mashhadani, Asia H.; Baatout, Sarah; Jacquet, Paul (Peer reviewer).

In: European Journal of Scientific Research, Vol. 53, No. 3, 05.05.2011, p. 470-476.

Research output: Contribution to journalArticlepeer-review

Harvard

Faraj, KA, Elias, MM, Al-Mashhadani, AH, Baatout, S & Jacquet, P 2011, 'Effect of X- and Gamma Rays on DNA in Human Cells', European Journal of Scientific Research, vol. 53, no. 3, pp. 470-476.

APA

Faraj, K. A., Elias, M. M., Al-Mashhadani, A. H., Baatout, S., & Jacquet, P. (2011). Effect of X- and Gamma Rays on DNA in Human Cells. European Journal of Scientific Research, 53(3), 470-476.

Vancouver

Faraj KA, Elias MM, Al-Mashhadani AH, Baatout S, Jacquet P. Effect of X- and Gamma Rays on DNA in Human Cells. European Journal of Scientific Research. 2011 May 5;53(3):470-476.

Author

Faraj, Kharman A. ; Elias, Mazin M. ; Al-Mashhadani, Asia H. ; Baatout, Sarah ; Jacquet, Paul. / Effect of X- and Gamma Rays on DNA in Human Cells. In: European Journal of Scientific Research. 2011 ; Vol. 53, No. 3. pp. 470-476.

Bibtex - Download

@article{b158f2df657b4ba5a3834d04e19e7180,
title = "Effect of X- and Gamma Rays on DNA in Human Cells",
abstract = "The purpose of this study was to investigate the effect of different doses and dose rates of x-rays and Co-60 gamma rays on DNA in normal human mononuclear cells. The cells were irradiated with x-ray doses (0.25, 0.5, 1, 2, 4) Gy at dose rate 0.28 Gy/min or with doses (15.625, 31.25, 62.5, 125, 250) mGy at dose rate 20 mGy/min or with X-ray doses (0.25, 0.5, 1, 2, 4 Gy at dose rate 2Gy/hr. The amount of 8-oxoguanine were detected by fluorescein-isothiocyanate (FITC) labeled antibodies. Using flow cytometry, the number of 8-oxoguanine /cell was calculated by calibration using spheric particles. Significant damage of DNA was observed for both x and rays. We concluded that DNA damage depends on dose and dose rate, and it is good biomarker for radiation response.",
keywords = "X-irradiation, lymphocytes, apoptosis, DNA damage, 8-oxoG, flow cytometry",
author = "Faraj, {Kharman A.} and Elias, {Mazin M.} and Al-Mashhadani, {Asia H.} and Sarah Baatout and Paul Jacquet",
note = "Score = 10",
year = "2011",
month = may,
day = "5",
language = "English",
volume = "53",
pages = "470--476",
journal = "European Journal of Scientific Research",
issn = "1450-216X",
publisher = "European Journal of Scientific Research",
number = "3",

}

RIS - Download

TY - JOUR

T1 - Effect of X- and Gamma Rays on DNA in Human Cells

AU - Faraj, Kharman A.

AU - Elias, Mazin M.

AU - Al-Mashhadani, Asia H.

AU - Baatout, Sarah

A2 - Jacquet, Paul

N1 - Score = 10

PY - 2011/5/5

Y1 - 2011/5/5

N2 - The purpose of this study was to investigate the effect of different doses and dose rates of x-rays and Co-60 gamma rays on DNA in normal human mononuclear cells. The cells were irradiated with x-ray doses (0.25, 0.5, 1, 2, 4) Gy at dose rate 0.28 Gy/min or with doses (15.625, 31.25, 62.5, 125, 250) mGy at dose rate 20 mGy/min or with X-ray doses (0.25, 0.5, 1, 2, 4 Gy at dose rate 2Gy/hr. The amount of 8-oxoguanine were detected by fluorescein-isothiocyanate (FITC) labeled antibodies. Using flow cytometry, the number of 8-oxoguanine /cell was calculated by calibration using spheric particles. Significant damage of DNA was observed for both x and rays. We concluded that DNA damage depends on dose and dose rate, and it is good biomarker for radiation response.

AB - The purpose of this study was to investigate the effect of different doses and dose rates of x-rays and Co-60 gamma rays on DNA in normal human mononuclear cells. The cells were irradiated with x-ray doses (0.25, 0.5, 1, 2, 4) Gy at dose rate 0.28 Gy/min or with doses (15.625, 31.25, 62.5, 125, 250) mGy at dose rate 20 mGy/min or with X-ray doses (0.25, 0.5, 1, 2, 4 Gy at dose rate 2Gy/hr. The amount of 8-oxoguanine were detected by fluorescein-isothiocyanate (FITC) labeled antibodies. Using flow cytometry, the number of 8-oxoguanine /cell was calculated by calibration using spheric particles. Significant damage of DNA was observed for both x and rays. We concluded that DNA damage depends on dose and dose rate, and it is good biomarker for radiation response.

KW - X-irradiation

KW - lymphocytes

KW - apoptosis

KW - DNA damage

KW - 8-oxoG

KW - flow cytometry

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_113613

UR - http://knowledgecentre.sckcen.be/so2/bibref/8019

M3 - Article

VL - 53

SP - 470

EP - 476

JO - European Journal of Scientific Research

JF - European Journal of Scientific Research

SN - 1450-216X

IS - 3

ER -

ID: 165296