Effects of pH on uranium uptake and oxidative stress responses induced in Arabidopsis thaliana

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Effects of pH on uranium uptake and oxidative stress responses induced in Arabidopsis thaliana. / Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean; Vangronsveld, Jaco; Cuypers, Ann; Vanmarcke, Hans (Peer reviewer).

In: Environmental Toxicology and Chemistry, Vol. 32, No. 9, 06.2013, p. 2125-2133.

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@article{00f35f35bceb4e7d81bd7a0c870d2b2e,
title = "Effects of pH on uranium uptake and oxidative stress responses induced in Arabidopsis thaliana",
abstract = "Since it is unknown how different U species determine U uptake and translocation within plants and how they might affect the oxidative defence mechanisms of these plants, this study aimed to analyse U uptake and oxidative stress related responses of different U species in Arabidopsis thaliana plants (Columbia ecotype). Eighteen-day-old seedlings were exposed for three days to 25 µM U in a Hoagland nutrient solution of which the pH was adjusted to 4.5, 5.5, 6.5 or 7.5. Results indicate that there is a different rate in U uptake and translocation at the different pHs, with high uptake and low translocation at low pH and lower uptake but higher translocation at high pH. After U exposure, an increased glutathione reductase activity and total glutathione concentration were observed in U-exposed roots, pointing towards an important role for glutathione in the root defence system against U either by chelation (phytochelatin production) or antioxidative defence mechanisms (cellular redox state). In the leaves, the antioxidative defence mechanisms were activated upon U exposure, indicated by an increased superoxide dismutase and catalase activity.",
keywords = "uranium, Arabidopsis thaliana, oxidative stress, pH",
author = "Eline Saenen and Nele Horemans and Nathalie Vanhoudt and Hildegarde Vandenhove and Geert Biermans and {Van Hees}, May and Jean Wannijn and Jaco Vangronsveld and Ann Cuypers and Hans Vanmarcke",
note = "Score = 10",
year = "2013",
month = "6",
doi = "10.1002/etc.2290",
language = "English",
volume = "32",
pages = "2125--2133",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "Wiley - John Wiley & Sons, Ltd",
number = "9",

}

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TY - JOUR

T1 - Effects of pH on uranium uptake and oxidative stress responses induced in Arabidopsis thaliana

AU - Saenen, Eline

AU - Horemans, Nele

AU - Vanhoudt, Nathalie

AU - Vandenhove, Hildegarde

AU - Biermans, Geert

AU - Van Hees, May

AU - Wannijn, Jean

AU - Vangronsveld, Jaco

AU - Cuypers, Ann

A2 - Vanmarcke, Hans

N1 - Score = 10

PY - 2013/6

Y1 - 2013/6

N2 - Since it is unknown how different U species determine U uptake and translocation within plants and how they might affect the oxidative defence mechanisms of these plants, this study aimed to analyse U uptake and oxidative stress related responses of different U species in Arabidopsis thaliana plants (Columbia ecotype). Eighteen-day-old seedlings were exposed for three days to 25 µM U in a Hoagland nutrient solution of which the pH was adjusted to 4.5, 5.5, 6.5 or 7.5. Results indicate that there is a different rate in U uptake and translocation at the different pHs, with high uptake and low translocation at low pH and lower uptake but higher translocation at high pH. After U exposure, an increased glutathione reductase activity and total glutathione concentration were observed in U-exposed roots, pointing towards an important role for glutathione in the root defence system against U either by chelation (phytochelatin production) or antioxidative defence mechanisms (cellular redox state). In the leaves, the antioxidative defence mechanisms were activated upon U exposure, indicated by an increased superoxide dismutase and catalase activity.

AB - Since it is unknown how different U species determine U uptake and translocation within plants and how they might affect the oxidative defence mechanisms of these plants, this study aimed to analyse U uptake and oxidative stress related responses of different U species in Arabidopsis thaliana plants (Columbia ecotype). Eighteen-day-old seedlings were exposed for three days to 25 µM U in a Hoagland nutrient solution of which the pH was adjusted to 4.5, 5.5, 6.5 or 7.5. Results indicate that there is a different rate in U uptake and translocation at the different pHs, with high uptake and low translocation at low pH and lower uptake but higher translocation at high pH. After U exposure, an increased glutathione reductase activity and total glutathione concentration were observed in U-exposed roots, pointing towards an important role for glutathione in the root defence system against U either by chelation (phytochelatin production) or antioxidative defence mechanisms (cellular redox state). In the leaves, the antioxidative defence mechanisms were activated upon U exposure, indicated by an increased superoxide dismutase and catalase activity.

KW - uranium

KW - Arabidopsis thaliana

KW - oxidative stress

KW - pH

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131148

UR - http://knowledgecentre.sckcen.be/so2/bibref/10559

U2 - 10.1002/etc.2290

DO - 10.1002/etc.2290

M3 - Article

VL - 32

SP - 2125

EP - 2133

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 9

ER -

ID: 97010