Effects of simulated space conditions on foetal mouse fibroblasts

Research output: Contribution to journalArticle

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Effects of simulated space conditions on foetal mouse fibroblasts. / Beck, Michaël; Hautefelt, Julien; Jacquet, Paul; Derradji, Hanane; van Oostveldt, Kaat; Buset, Jasmine; Neefs, Mieke; Vanhavere, Filip; van Oostveldt, Patrick; Baatout, Sarah.

In: Communications in agricultural and applied biological sciences, Vol. 73, No. 1, 09.2008, p. 103-107.

Research output: Contribution to journalArticle

Harvard

Beck, M, Hautefelt, J, Jacquet, P, Derradji, H, van Oostveldt, K, Buset, J, Neefs, M, Vanhavere, F, van Oostveldt, P & Baatout, S 2008, 'Effects of simulated space conditions on foetal mouse fibroblasts', Communications in agricultural and applied biological sciences, vol. 73, no. 1, pp. 103-107.

APA

Beck, M., Hautefelt, J., Jacquet, P., Derradji, H., van Oostveldt, K., Buset, J., ... Baatout, S. (2008). Effects of simulated space conditions on foetal mouse fibroblasts. Communications in agricultural and applied biological sciences, 73(1), 103-107.

Vancouver

Beck M, Hautefelt J, Jacquet P, Derradji H, van Oostveldt K, Buset J et al. Effects of simulated space conditions on foetal mouse fibroblasts. Communications in agricultural and applied biological sciences. 2008 Sep;73(1):103-107.

Author

Beck, Michaël ; Hautefelt, Julien ; Jacquet, Paul ; Derradji, Hanane ; van Oostveldt, Kaat ; Buset, Jasmine ; Neefs, Mieke ; Vanhavere, Filip ; van Oostveldt, Patrick ; Baatout, Sarah. / Effects of simulated space conditions on foetal mouse fibroblasts. In: Communications in agricultural and applied biological sciences. 2008 ; Vol. 73, No. 1. pp. 103-107.

Bibtex - Download

@article{25d11bdbe2d5404ab797c7702e64289f,
title = "Effects of simulated space conditions on foetal mouse fibroblasts",
abstract = "Radiation and microgravity are amongst the most important stresses in space conditions. Gravity is universally present on Earth. It determines the vertical orientation of all living organisms, thus their proper development. As a matter of fact, the presence of gravity at critical developmental moments of life is likely to determine the morphology that subsequently develops. It has a major impact on many biological functions, from muscle properties and contractile function to primary gravity transducer, as well as on neuronal signals and controls, behaviour, etc. The purpose of this study is to decipher the effects of simulated space conditions (microgravity and irradiation) on the morphology, physiology and gene expression of the mouse foetus. The developmental period that has been selected within this study is the late organogenesis. Embryonic fibroblast primary cell cultures were performed on foetuses at day 19 post conceptio and cultures were then submitted to microgravity and/or X-radiation conditions. To simulate microgravity, the desktop Random Positioning Machine (RPM) was used. The STO cell line was used to optimise and set-up experimental conditions. Flow cytometry was then applied to quantify possible space-induced apoptosis as well as potential effect on the cell cycle. Cytospin analyses were performed for morphology assessments.",
keywords = "mouse development, fibroblasts, space conditions, microgravity, fetus, radiation",
author = "Micha{\"e}l Beck and Julien Hautefelt and Paul Jacquet and Hanane Derradji and {van Oostveldt}, Kaat and Jasmine Buset and Mieke Neefs and Filip Vanhavere and {van Oostveldt}, Patrick and Sarah Baatout",
note = "Score = 2",
year = "2008",
month = "9",
language = "English",
volume = "73",
pages = "103--107",
journal = "Communications in agricultural and applied biological sciences",
issn = "1379-1176",
publisher = "UGent - Universiteit Gent",
number = "1",

}

RIS - Download

TY - JOUR

T1 - Effects of simulated space conditions on foetal mouse fibroblasts

AU - Beck, Michaël

AU - Hautefelt, Julien

AU - Jacquet, Paul

AU - Derradji, Hanane

AU - van Oostveldt, Kaat

AU - Buset, Jasmine

AU - Neefs, Mieke

AU - Vanhavere, Filip

AU - van Oostveldt, Patrick

AU - Baatout, Sarah

N1 - Score = 2

PY - 2008/9

Y1 - 2008/9

N2 - Radiation and microgravity are amongst the most important stresses in space conditions. Gravity is universally present on Earth. It determines the vertical orientation of all living organisms, thus their proper development. As a matter of fact, the presence of gravity at critical developmental moments of life is likely to determine the morphology that subsequently develops. It has a major impact on many biological functions, from muscle properties and contractile function to primary gravity transducer, as well as on neuronal signals and controls, behaviour, etc. The purpose of this study is to decipher the effects of simulated space conditions (microgravity and irradiation) on the morphology, physiology and gene expression of the mouse foetus. The developmental period that has been selected within this study is the late organogenesis. Embryonic fibroblast primary cell cultures were performed on foetuses at day 19 post conceptio and cultures were then submitted to microgravity and/or X-radiation conditions. To simulate microgravity, the desktop Random Positioning Machine (RPM) was used. The STO cell line was used to optimise and set-up experimental conditions. Flow cytometry was then applied to quantify possible space-induced apoptosis as well as potential effect on the cell cycle. Cytospin analyses were performed for morphology assessments.

AB - Radiation and microgravity are amongst the most important stresses in space conditions. Gravity is universally present on Earth. It determines the vertical orientation of all living organisms, thus their proper development. As a matter of fact, the presence of gravity at critical developmental moments of life is likely to determine the morphology that subsequently develops. It has a major impact on many biological functions, from muscle properties and contractile function to primary gravity transducer, as well as on neuronal signals and controls, behaviour, etc. The purpose of this study is to decipher the effects of simulated space conditions (microgravity and irradiation) on the morphology, physiology and gene expression of the mouse foetus. The developmental period that has been selected within this study is the late organogenesis. Embryonic fibroblast primary cell cultures were performed on foetuses at day 19 post conceptio and cultures were then submitted to microgravity and/or X-radiation conditions. To simulate microgravity, the desktop Random Positioning Machine (RPM) was used. The STO cell line was used to optimise and set-up experimental conditions. Flow cytometry was then applied to quantify possible space-induced apoptosis as well as potential effect on the cell cycle. Cytospin analyses were performed for morphology assessments.

KW - mouse development

KW - fibroblasts

KW - space conditions

KW - microgravity

KW - fetus

KW - radiation

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_91442

UR - http://knowledgecentre.sckcen.be/so2/bibref/5152

M3 - Article

VL - 73

SP - 103

EP - 107

JO - Communications in agricultural and applied biological sciences

JF - Communications in agricultural and applied biological sciences

SN - 1379-1176

IS - 1

ER -

ID: 75376