EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology

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EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology. / Vandevoorde, Charlot; Gomolka, Maria; Roessler, Ute; Samaga, Daniel; Lindholm, Carita; Fernet, Marie; Hall, Janet; Pernot, Eileen; El Saghire, Houssein; Baatout, Sarah; Kesminiene, Ausrele; Thierens, Hubert; Vanmarcke, Hans (Peer reviewer); Quintens, Roel (Peer reviewer).

In: International Journal of Radiation Biology, Vol. 91, No. 8, 08.2015, p. 653-663.

Research output: Contribution to journalArticlepeer-review

Harvard

Vandevoorde, C, Gomolka, M, Roessler, U, Samaga, D, Lindholm, C, Fernet, M, Hall, J, Pernot, E, El Saghire, H, Baatout, S, Kesminiene, A, Thierens, H, Vanmarcke, H & Quintens, R 2015, 'EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology', International Journal of Radiation Biology, vol. 91, no. 8, pp. 653-663. https://doi.org/10.3109/09553002.2015.1047987

APA

Vandevoorde, C., Gomolka, M., Roessler, U., Samaga, D., Lindholm, C., Fernet, M., Hall, J., Pernot, E., El Saghire, H., Baatout, S., Kesminiene, A., Thierens, H., Vanmarcke, H., & Quintens, R. (2015). EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology. International Journal of Radiation Biology, 91(8), 653-663. https://doi.org/10.3109/09553002.2015.1047987

Vancouver

Vandevoorde C, Gomolka M, Roessler U, Samaga D, Lindholm C, Fernet M et al. EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology. International Journal of Radiation Biology. 2015 Aug;91(8):653-663. https://doi.org/10.3109/09553002.2015.1047987

Author

Vandevoorde, Charlot ; Gomolka, Maria ; Roessler, Ute ; Samaga, Daniel ; Lindholm, Carita ; Fernet, Marie ; Hall, Janet ; Pernot, Eileen ; El Saghire, Houssein ; Baatout, Sarah ; Kesminiene, Ausrele ; Thierens, Hubert ; Vanmarcke, Hans ; Quintens, Roel. / EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology. In: International Journal of Radiation Biology. 2015 ; Vol. 91, No. 8. pp. 653-663.

Bibtex - Download

@article{8a3722bce5644b7aa40fe7d7669ead94,
title = "EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology",
abstract = "Purpose : To conduct a feasibility study on the application of the gamma-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting. A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting, processing and storing of blood samples the reliability of foci scoring for low doses, as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments. In order to test the feasibility of pooling the gamma-H2AX data when different centres are involved in an international multicentre study, two intercomparison studies in the low-dose range (10 – 500 mGy) were performed.",
keywords = "gamma-H2AX foci, computed tomography, DNA damage, molecular radiobiology, low dose effects, epidemiology",
author = "Charlot Vandevoorde and Maria Gomolka and Ute Roessler and Daniel Samaga and Carita Lindholm and Marie Fernet and Janet Hall and Eileen Pernot and {El Saghire}, Houssein and Sarah Baatout and Ausrele Kesminiene and Hubert Thierens and Hans Vanmarcke and Roel Quintens",
note = "Score = 10",
year = "2015",
month = aug,
doi = "10.3109/09553002.2015.1047987",
language = "English",
volume = "91",
pages = "653--663",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Taylor & Francis (CRC)",
number = "8",

}

RIS - Download

TY - JOUR

T1 - EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology

AU - Vandevoorde, Charlot

AU - Gomolka, Maria

AU - Roessler, Ute

AU - Samaga, Daniel

AU - Lindholm, Carita

AU - Fernet, Marie

AU - Hall, Janet

AU - Pernot, Eileen

AU - El Saghire, Houssein

AU - Baatout, Sarah

AU - Kesminiene, Ausrele

AU - Thierens, Hubert

A2 - Vanmarcke, Hans

A2 - Quintens, Roel

N1 - Score = 10

PY - 2015/8

Y1 - 2015/8

N2 - Purpose : To conduct a feasibility study on the application of the gamma-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting. A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting, processing and storing of blood samples the reliability of foci scoring for low doses, as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments. In order to test the feasibility of pooling the gamma-H2AX data when different centres are involved in an international multicentre study, two intercomparison studies in the low-dose range (10 – 500 mGy) were performed.

AB - Purpose : To conduct a feasibility study on the application of the gamma-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting. A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting, processing and storing of blood samples the reliability of foci scoring for low doses, as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments. In order to test the feasibility of pooling the gamma-H2AX data when different centres are involved in an international multicentre study, two intercomparison studies in the low-dose range (10 – 500 mGy) were performed.

KW - gamma-H2AX foci

KW - computed tomography

KW - DNA damage

KW - molecular radiobiology

KW - low dose effects

KW - epidemiology

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140810

UR - http://knowledgecentre.sckcen.be/so2/bibref/13048

U2 - 10.3109/09553002.2015.1047987

DO - 10.3109/09553002.2015.1047987

M3 - Article

VL - 91

SP - 653

EP - 663

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 8

ER -

ID: 161505