Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

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Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight. / Mastroleo, Felice; Van Houdt, Rob; Leroy, Baptiste; Benotmane, Rafi; Janssen, Ann; Mergeay, Max; Vanhavere, Filip; Hendrickx, Larissa; Wattiez, Ruddy; Leys, Natalie; Monsieurs, Pieter (Peer reviewer).

In: The ISME Journal: Multidisciplinary Journal of Microbial Ecology, Vol. 3, No. 12, 02.07.2009, p. 1402-1419.

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@article{854b6e9986634415b109880508c1bc43,
title = "Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight",
abstract = "In the frame of the MELiSSA project from the European Space Agency, the alpha-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission",
keywords = "ionizing radiation, MELiSSA, random positioning machine, Rhodospirillum rubrum, space flight",
author = "Felice Mastroleo and {Van Houdt}, Rob and Baptiste Leroy and Rafi Benotmane and Ann Janssen and Max Mergeay and Filip Vanhavere and Larissa Hendrickx and Ruddy Wattiez and Natalie Leys and Pieter Monsieurs",
note = "Score = 10",
year = "2009",
month = "7",
day = "2",
doi = "10.1038/ismej.2009.74",
language = "English",
volume = "3",
pages = "1402--1419",
journal = "The ISME Journal: Multidisciplinary Journal of Microbial Ecology",
issn = "1751-7362",
publisher = "Nature Publishing Group",
number = "12",

}

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TY - JOUR

T1 - Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

AU - Mastroleo, Felice

AU - Van Houdt, Rob

AU - Leroy, Baptiste

AU - Benotmane, Rafi

AU - Janssen, Ann

AU - Mergeay, Max

AU - Vanhavere, Filip

AU - Hendrickx, Larissa

AU - Wattiez, Ruddy

AU - Leys, Natalie

A2 - Monsieurs, Pieter

N1 - Score = 10

PY - 2009/7/2

Y1 - 2009/7/2

N2 - In the frame of the MELiSSA project from the European Space Agency, the alpha-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission

AB - In the frame of the MELiSSA project from the European Space Agency, the alpha-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission

KW - ionizing radiation

KW - MELiSSA

KW - random positioning machine

KW - Rhodospirillum rubrum

KW - space flight

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_101219

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_101219_2

UR - http://knowledgecentre.sckcen.be/so2/bibref/6226

U2 - 10.1038/ismej.2009.74

DO - 10.1038/ismej.2009.74

M3 - Article

VL - 3

SP - 1402

EP - 1419

JO - The ISME Journal: Multidisciplinary Journal of Microbial Ecology

JF - The ISME Journal: Multidisciplinary Journal of Microbial Ecology

SN - 1751-7362

IS - 12

ER -

ID: 368984