GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity

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GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity. / Basile Carballo, Gabriela; Ribeiro, Jessica; Pinto de la Faria Lopes, Giselle; Pereira Ferrer, Valéria; Sperduto Dezonne, Romulo; Pereira, Cláudia Maria; Leite de Sampaio e Spohr, Tania Cristina.

In: Cellular and Molecular Neurobiology, 13.02.2020, p. 1-18.

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Harvard

Basile Carballo, G, Ribeiro, J, Pinto de la Faria Lopes, G, Pereira Ferrer, V, Sperduto Dezonne, R, Pereira, CM & Leite de Sampaio e Spohr, TC 2020, 'GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity', Cellular and Molecular Neurobiology, pp. 1-18. https://doi.org/10.1007%2Fs10571-020-00891-6

APA

Basile Carballo, G., Ribeiro, J., Pinto de la Faria Lopes, G., Pereira Ferrer, V., Sperduto Dezonne, R., Pereira, C. M., & Leite de Sampaio e Spohr, T. C. (2020). GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity. Cellular and Molecular Neurobiology, 1-18. https://doi.org/10.1007%2Fs10571-020-00891-6

Vancouver

Basile Carballo G, Ribeiro J, Pinto de la Faria Lopes G, Pereira Ferrer V, Sperduto Dezonne R, Pereira CM et al. GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity. Cellular and Molecular Neurobiology. 2020 Feb 13;1-18. https://doi.org/10.1007%2Fs10571-020-00891-6

Author

Basile Carballo, Gabriela ; Ribeiro, Jessica ; Pinto de la Faria Lopes, Giselle ; Pereira Ferrer, Valéria ; Sperduto Dezonne, Romulo ; Pereira, Cláudia Maria ; Leite de Sampaio e Spohr, Tania Cristina. / GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity. In: Cellular and Molecular Neurobiology. 2020 ; pp. 1-18.

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@article{a7892e4598df405988e0a10fdea1e016,
title = "GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity",
abstract = "Glioblastoma (GBM) is the most common adult primary tumor of the CNS characterized by rapid growth and diffuse invasiveness into the brain parenchyma. The GBM resistance to chemotherapeutic drugs may be due to the presence of cancer stem cells (CSCs). The CSCs activate the same molecular pathways as healthy stem cells such as WNT, Sonic hedgehog (SHH), and Notch. Mutations or deregulations of those pathways play a key role in the proliferation and differentiation of their surrounding environment, leading to tumorigenesis. Here we investigated the effect of SHH signaling pathway inhibition in human GBM cells by using GANT-61, considering stem cell phenotype, cell proliferation, and cell death. Our results demonstrated that GANT-61 induces apoptosis and autophagy in GBM cells, by increasing the expression of LC3 II and cleaved caspase 3 and 9. Moreover, we observed that SHH signaling plays a crucial role in CSC phenotype maintenance, being also involved in the epithelial-mesenchymal transition (EMT) phenotype. We also noted that SHH pathway modulation can regulate cell proliferation as revealed through the analysis of Ki-67 and c-MYC expressions. We concluded that SHH signaling pathway inhibition may be a promising therapeutic approach to treat patients suffering from GBM refractory to traditional treatments.",
keywords = "Sonic hedgehog, Glioblastoma, Cell death, Cell viability",
author = "{Basile Carballo}, Gabriela and Jessica Ribeiro and {Pinto de la Faria Lopes}, Giselle and {Pereira Ferrer}, Val{\'e}ria and {Sperduto Dezonne}, Romulo and Pereira, {Cl{\'a}udia Maria} and {Leite de Sampaio e Spohr}, {Tania Cristina}",
note = "Score=10",
year = "2020",
month = "2",
day = "13",
doi = "10.1007{\%}2Fs10571-020-00891-6",
language = "English",
pages = "1--18",
journal = "Cellular and Molecular Neurobiology",
issn = "0272-4340",
publisher = "Springer",

}

RIS - Download

TY - JOUR

T1 - GANT‑61 Induces Autophagy and Apoptosis in Glioblastoma Cells despite their heterogeneity

AU - Basile Carballo, Gabriela

AU - Ribeiro, Jessica

AU - Pinto de la Faria Lopes, Giselle

AU - Pereira Ferrer, Valéria

AU - Sperduto Dezonne, Romulo

AU - Pereira, Cláudia Maria

AU - Leite de Sampaio e Spohr, Tania Cristina

N1 - Score=10

PY - 2020/2/13

Y1 - 2020/2/13

N2 - Glioblastoma (GBM) is the most common adult primary tumor of the CNS characterized by rapid growth and diffuse invasiveness into the brain parenchyma. The GBM resistance to chemotherapeutic drugs may be due to the presence of cancer stem cells (CSCs). The CSCs activate the same molecular pathways as healthy stem cells such as WNT, Sonic hedgehog (SHH), and Notch. Mutations or deregulations of those pathways play a key role in the proliferation and differentiation of their surrounding environment, leading to tumorigenesis. Here we investigated the effect of SHH signaling pathway inhibition in human GBM cells by using GANT-61, considering stem cell phenotype, cell proliferation, and cell death. Our results demonstrated that GANT-61 induces apoptosis and autophagy in GBM cells, by increasing the expression of LC3 II and cleaved caspase 3 and 9. Moreover, we observed that SHH signaling plays a crucial role in CSC phenotype maintenance, being also involved in the epithelial-mesenchymal transition (EMT) phenotype. We also noted that SHH pathway modulation can regulate cell proliferation as revealed through the analysis of Ki-67 and c-MYC expressions. We concluded that SHH signaling pathway inhibition may be a promising therapeutic approach to treat patients suffering from GBM refractory to traditional treatments.

AB - Glioblastoma (GBM) is the most common adult primary tumor of the CNS characterized by rapid growth and diffuse invasiveness into the brain parenchyma. The GBM resistance to chemotherapeutic drugs may be due to the presence of cancer stem cells (CSCs). The CSCs activate the same molecular pathways as healthy stem cells such as WNT, Sonic hedgehog (SHH), and Notch. Mutations or deregulations of those pathways play a key role in the proliferation and differentiation of their surrounding environment, leading to tumorigenesis. Here we investigated the effect of SHH signaling pathway inhibition in human GBM cells by using GANT-61, considering stem cell phenotype, cell proliferation, and cell death. Our results demonstrated that GANT-61 induces apoptosis and autophagy in GBM cells, by increasing the expression of LC3 II and cleaved caspase 3 and 9. Moreover, we observed that SHH signaling plays a crucial role in CSC phenotype maintenance, being also involved in the epithelial-mesenchymal transition (EMT) phenotype. We also noted that SHH pathway modulation can regulate cell proliferation as revealed through the analysis of Ki-67 and c-MYC expressions. We concluded that SHH signaling pathway inhibition may be a promising therapeutic approach to treat patients suffering from GBM refractory to traditional treatments.

KW - Sonic hedgehog

KW - Glioblastoma

KW - Cell death

KW - Cell viability

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/overview/41738980

U2 - 10.1007%2Fs10571-020-00891-6

DO - 10.1007%2Fs10571-020-00891-6

M3 - Article

SP - 1

EP - 18

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

SN - 0272-4340

ER -

ID: 6993447