Gene expression changes in mouse fetal fibroblasts after chronic exposure to simulated space conditions

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Gene expression changes in mouse fetal fibroblasts after chronic exposure to simulated space conditions. / Beck, Michaël; Moreels, Marjan; Quintens, Roel; Abouelaradat, Khalil; Tabury, Kevin; Michaux, Arlette; Janssen, Ann; Neefs, Mieke; Van Oostveldt, Patrick; De Vos, Winnok H.; Baatout, Sarah.

Proceedings ENC 2012, European Nuclear Conference. Belgium, 2012. p. 210-215.

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Beck, M, Moreels, M, Quintens, R, Abouelaradat, K, Tabury, K, Michaux, A, Janssen, A, Neefs, M, Van Oostveldt, P, De Vos, WH & Baatout, S 2012, Gene expression changes in mouse fetal fibroblasts after chronic exposure to simulated space conditions. in Proceedings ENC 2012, European Nuclear Conference. Belgium, pp. 210-215, The European Forum to discuss Nuclear Technology Issues, Opportunities & Challenges - ENC 2012, Manchester, United Kingdom, 2012-12-09.

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@inproceedings{629f290031d94afa986187184f52a999,
title = "Gene expression changes in mouse fetal fibroblasts after chronic exposure to simulated space conditions",
abstract = "Space conditions including microgravity and cosmic radiation are difficult to simulate on Earth. However, ground-based models exist to simulate space flight conditions including irradiation (IR) facilities and µg simulating devices such as the Random Positioning Machine (RPM). In this study, a space simulating experimental model was used to monitor gene expression changes in primary skin fibroblasts obtained from mouse fetus. Cells were continuously exposed (65h) to a low dose (55mSv) IR mixture of neutrons and γ-rays and/or simulated microgravity (RPM), after which whole genome microarrays were performed. Two different analytical methods to detect changes in gene expression were used in parallel in this study including 'single gene analysis' and 'Gene Set Enrichment Analysis'. Our results show that simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes and decreasing the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low dose IR caused a down-regulation of genes involved in cytoskeleton pathways, but also in genes involved in cell cycle regulation and DNA damage response. Many of the genes or gene sets that were altered in the individual treatments were not significantly changed in the combined treatment, indicating no synergistic effect between RPM and IR.",
keywords = "cosmic radiation, gene expression changes, microgravity",
author = "Micha{\"e}l Beck and Marjan Moreels and Roel Quintens and Khalil Abouelaradat and Kevin Tabury and Arlette Michaux and Ann Janssen and Mieke Neefs and {Van Oostveldt}, Patrick and {De Vos}, {Winnok H.} and Sarah Baatout",
note = "Score = 3",
year = "2012",
month = "12",
language = "English",
pages = "210--215",
booktitle = "Proceedings ENC 2012, European Nuclear Conference",

}

RIS - Download

TY - GEN

T1 - Gene expression changes in mouse fetal fibroblasts after chronic exposure to simulated space conditions

AU - Beck, Michaël

AU - Moreels, Marjan

AU - Quintens, Roel

AU - Abouelaradat, Khalil

AU - Tabury, Kevin

AU - Michaux, Arlette

AU - Janssen, Ann

AU - Neefs, Mieke

AU - Van Oostveldt, Patrick

AU - De Vos, Winnok H.

AU - Baatout, Sarah

N1 - Score = 3

PY - 2012/12

Y1 - 2012/12

N2 - Space conditions including microgravity and cosmic radiation are difficult to simulate on Earth. However, ground-based models exist to simulate space flight conditions including irradiation (IR) facilities and µg simulating devices such as the Random Positioning Machine (RPM). In this study, a space simulating experimental model was used to monitor gene expression changes in primary skin fibroblasts obtained from mouse fetus. Cells were continuously exposed (65h) to a low dose (55mSv) IR mixture of neutrons and γ-rays and/or simulated microgravity (RPM), after which whole genome microarrays were performed. Two different analytical methods to detect changes in gene expression were used in parallel in this study including 'single gene analysis' and 'Gene Set Enrichment Analysis'. Our results show that simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes and decreasing the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low dose IR caused a down-regulation of genes involved in cytoskeleton pathways, but also in genes involved in cell cycle regulation and DNA damage response. Many of the genes or gene sets that were altered in the individual treatments were not significantly changed in the combined treatment, indicating no synergistic effect between RPM and IR.

AB - Space conditions including microgravity and cosmic radiation are difficult to simulate on Earth. However, ground-based models exist to simulate space flight conditions including irradiation (IR) facilities and µg simulating devices such as the Random Positioning Machine (RPM). In this study, a space simulating experimental model was used to monitor gene expression changes in primary skin fibroblasts obtained from mouse fetus. Cells were continuously exposed (65h) to a low dose (55mSv) IR mixture of neutrons and γ-rays and/or simulated microgravity (RPM), after which whole genome microarrays were performed. Two different analytical methods to detect changes in gene expression were used in parallel in this study including 'single gene analysis' and 'Gene Set Enrichment Analysis'. Our results show that simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes and decreasing the expression of genes involved in cytoskeleton remodeling. Similarly, chronic exposure to low dose IR caused a down-regulation of genes involved in cytoskeleton pathways, but also in genes involved in cell cycle regulation and DNA damage response. Many of the genes or gene sets that were altered in the individual treatments were not significantly changed in the combined treatment, indicating no synergistic effect between RPM and IR.

KW - cosmic radiation

KW - gene expression changes

KW - microgravity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_129000

UR - http://knowledgecentre.sckcen.be/so2/bibref/10259

M3 - In-proceedings paper

SP - 210

EP - 215

BT - Proceedings ENC 2012, European Nuclear Conference

CY - Belgium

ER -

ID: 123833