Abstract
Health risks from exposure to low doses of ionizing
radiation (IR) are becoming a concern due to the rapidly growing
medical applications of X-rays. Using microarray techniques, this
study aims for a better understanding of whole blood response
to low and high doses of IR. Functional analysis of genes diff erentially expressed at 0.05 Gy showed the enrichment of chemokine and cytokine
signaling. However, responsive genes to 1 Gy were mainly
involved in tumor suppressor protein 53 (p53) pathways. In a
second approach, GSEA showed a higher statistical ranking of
inflammatory and immune-related gene sets that are involved
in both responding and/or secretion of growth factors, chemokines,
and cytokines. This indicates the activation of the immune
response. Whereas, gene sets enriched at 1 Gy were ‘ classical ’
radiation pathways like p53 signaling, apoptosis, DNA damage
and repair. Comparative RT-PCR studies showed the signifi cant
induction of chemokine-related genes (PF4 , GNG11 and CCR4)
at 0.05 Gy and DNA damage and repair genes at 1 Gy (DDB2 ,
AEN and CDKN1A).
Details
Original language | English |
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Pages (from-to) | 628-638 |
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Journal | International Journal of Radiation Biology |
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Volume | 89 |
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Issue number | 8 |
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DOIs | |
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Publication status | Published - Aug 2013 |
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