Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses

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Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses. / El Saghire, Houssein; Thierens, Hubert; Monsieurs, Pieter; Michaux, Arlette; Vandevoorde, Charlot; Baatout, Sarah.

In: International Journal of Radiation Biology, Vol. 89, No. 8, 08.2013, p. 628-638.

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@article{2036dc4d8f164c98b195f56ea06df1a7,
title = "Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses",
abstract = "Health risks from exposure to low doses of ionizing radiation (IR) are becoming a concern due to the rapidly growing medical applications of X-rays. Using microarray techniques, this study aims for a better understanding of whole blood response to low and high doses of IR. Functional analysis of genes diff erentially expressed at 0.05 Gy showed the enrichment of chemokine and cytokine signaling. However, responsive genes to 1 Gy were mainly involved in tumor suppressor protein 53 (p53) pathways. In a second approach, GSEA showed a higher statistical ranking of inflammatory and immune-related gene sets that are involved in both responding and/or secretion of growth factors, chemokines, and cytokines. This indicates the activation of the immune response. Whereas, gene sets enriched at 1 Gy were {\textquoteleft} classical {\textquoteright} radiation pathways like p53 signaling, apoptosis, DNA damage and repair. Comparative RT-PCR studies showed the signifi cant induction of chemokine-related genes (PF4 , GNG11 and CCR4) at 0.05 Gy and DNA damage and repair genes at 1 Gy (DDB2 , AEN and CDKN1A).",
keywords = "microarray, low dose, high dose, ionizing radiation, whole blood, GSEA",
author = "{El Saghire}, Houssein and Hubert Thierens and Pieter Monsieurs and Arlette Michaux and Charlot Vandevoorde and Sarah Baatout",
note = "Score = 10",
year = "2013",
month = aug,
doi = "10.3109/09553002.2013.782448",
language = "English",
volume = "89",
pages = "628--638",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Taylor & Francis (CRC)",
number = "8",

}

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TY - JOUR

T1 - Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses

AU - El Saghire, Houssein

AU - Thierens, Hubert

AU - Monsieurs, Pieter

AU - Michaux, Arlette

AU - Vandevoorde, Charlot

AU - Baatout, Sarah

N1 - Score = 10

PY - 2013/8

Y1 - 2013/8

N2 - Health risks from exposure to low doses of ionizing radiation (IR) are becoming a concern due to the rapidly growing medical applications of X-rays. Using microarray techniques, this study aims for a better understanding of whole blood response to low and high doses of IR. Functional analysis of genes diff erentially expressed at 0.05 Gy showed the enrichment of chemokine and cytokine signaling. However, responsive genes to 1 Gy were mainly involved in tumor suppressor protein 53 (p53) pathways. In a second approach, GSEA showed a higher statistical ranking of inflammatory and immune-related gene sets that are involved in both responding and/or secretion of growth factors, chemokines, and cytokines. This indicates the activation of the immune response. Whereas, gene sets enriched at 1 Gy were ‘ classical ’ radiation pathways like p53 signaling, apoptosis, DNA damage and repair. Comparative RT-PCR studies showed the signifi cant induction of chemokine-related genes (PF4 , GNG11 and CCR4) at 0.05 Gy and DNA damage and repair genes at 1 Gy (DDB2 , AEN and CDKN1A).

AB - Health risks from exposure to low doses of ionizing radiation (IR) are becoming a concern due to the rapidly growing medical applications of X-rays. Using microarray techniques, this study aims for a better understanding of whole blood response to low and high doses of IR. Functional analysis of genes diff erentially expressed at 0.05 Gy showed the enrichment of chemokine and cytokine signaling. However, responsive genes to 1 Gy were mainly involved in tumor suppressor protein 53 (p53) pathways. In a second approach, GSEA showed a higher statistical ranking of inflammatory and immune-related gene sets that are involved in both responding and/or secretion of growth factors, chemokines, and cytokines. This indicates the activation of the immune response. Whereas, gene sets enriched at 1 Gy were ‘ classical ’ radiation pathways like p53 signaling, apoptosis, DNA damage and repair. Comparative RT-PCR studies showed the signifi cant induction of chemokine-related genes (PF4 , GNG11 and CCR4) at 0.05 Gy and DNA damage and repair genes at 1 Gy (DDB2 , AEN and CDKN1A).

KW - microarray

KW - low dose

KW - high dose

KW - ionizing radiation

KW - whole blood

KW - GSEA

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131735

UR - http://knowledgecentre.sckcen.be/so2/bibref/10681

U2 - 10.3109/09553002.2013.782448

DO - 10.3109/09553002.2013.782448

M3 - Article

VL - 89

SP - 628

EP - 638

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 8

ER -

ID: 352212