Genome-wide DNA methylation changes in two Brassicaceae species sampled alongside a radiation gradient in Chernobyl and Fukushima

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Genome-wide DNA methylation changes in two Brassicaceae species sampled alongside a radiation gradient in Chernobyl and Fukushima. / Horemans, Nele; Nauts, Robin; Vives i Batlle, Jordi; Van Hees, May; Jacobs, G.; Voorspoels, Stefan ; Gaschak, Sergei; Nanba, Kenji; Saenen, Eline.

In: Journal of environmental radioactivity, Vol. 192, 01.12.2018, p. 405–416.

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Horemans, Nele ; Nauts, Robin ; Vives i Batlle, Jordi ; Van Hees, May ; Jacobs, G. ; Voorspoels, Stefan ; Gaschak, Sergei ; Nanba, Kenji ; Saenen, Eline. / Genome-wide DNA methylation changes in two Brassicaceae species sampled alongside a radiation gradient in Chernobyl and Fukushima. In: Journal of environmental radioactivity. 2018 ; Vol. 192. pp. 405–416.

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@article{d0464cf6ee9c42ef8950701bdff17976,
title = "Genome-wide DNA methylation changes in two Brassicaceae species sampled alongside a radiation gradient in Chernobyl and Fukushima",
abstract = "The long-term radiological impact to the environment of the nuclear accidents in Chernobyl and Fukushima is still under discussion. In the course of spring of 2016 we sampled two Brassicacea plants, Arabidopsis thaliana and Capsella bursa-pastoris native to Ukraine and Japan, respectively, alongside a gradient of radiation within the exclusion and difficult to return zones of Chernobyl (CEZ) and Fukushima (FEZ). Ambient dose rates were similar for both sampling gradients ranging from 0.5 to 80 μGy/h at plant height. The hypothesis was tested whether a history of several generations of plants growing in enhanced radiation exposure conditions would have led to changes in genome-wide DNA methylation. However, no differences were found in the global percentage of 5-methylated cytosines in Capsella bursa pastoris plants sampled in FEZ. On the other hand a significant decrease in whole genome methylation percentage in Arabidopsis thaliana plants was found in CEZ mainly governed by the highest exposed plants. These data support a link between exposure to changed environmental conditions and changes genome methylation. In addition to methylation the activity concentration of different radionuclides, 137Cs, 90Sr, 241Am and Pu-238,239,240 for CEZ and 137, 134Cs for FEZ, was analysed in both soil and plant samples. The ratio of 5.6 between 137Cs compared to 134Cs was as expected five years after the FEZ accident. For CEZ 137Cs is the most abundant polluting radionuclide in soil followed by 90Sr. Whereas 241Am and Pu-isotopes are only marginally present. In the plant tissue, however, higher levels of Sr than Cs were retrieved due to a high uptake of 90Sr in the plants. The 90Sr transfer factors ranged in CEZ from 5 to 20 (kg/kg) depending on the locality. Based on the activity concentrations of the different radionuclides the ERICA tool was used to estimate the total dose rates to the plants. It was found that for FEZ the doses was mainly contributable to the external Cs-isotopes and as such estimated total dose rates (0.13–38 μGy/h) were in the same range as the ambient measured dose rates. In strong contrast this was not true for CEZ where the total dose rate was mainly due to high uptake of the 90Sr leading to dose rates ranging from 1 to 370 μGy/h. Hence our data clearly indicate that not taking into account the internal contamination in CEZ will lead to considerable underestimation of the doses to the plants. Additionally they show that it is hard to compare the two nuclear accidental sites and one of the main reasons is the difference in contamination profile.",
keywords = "DNA hypermethylation, exposure, radiation",
author = "Nele Horemans and Robin Nauts and {Vives i Batlle}, Jordi and {Van Hees}, May and G. Jacobs and Stefan Voorspoels and Sergei Gaschak and Kenji Nanba and Eline Saenen",
note = "Score=10",
year = "2018",
month = "12",
day = "1",
doi = "10.1016/j.jenvrad.2018.07.012",
language = "English",
volume = "192",
pages = "405–416",
journal = "Journal of environmental radioactivity",
issn = "0265-931X",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Genome-wide DNA methylation changes in two Brassicaceae species sampled alongside a radiation gradient in Chernobyl and Fukushima

AU - Horemans, Nele

AU - Nauts, Robin

AU - Vives i Batlle, Jordi

AU - Van Hees, May

AU - Jacobs, G.

AU - Voorspoels, Stefan

AU - Gaschak, Sergei

AU - Nanba, Kenji

AU - Saenen, Eline

N1 - Score=10

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The long-term radiological impact to the environment of the nuclear accidents in Chernobyl and Fukushima is still under discussion. In the course of spring of 2016 we sampled two Brassicacea plants, Arabidopsis thaliana and Capsella bursa-pastoris native to Ukraine and Japan, respectively, alongside a gradient of radiation within the exclusion and difficult to return zones of Chernobyl (CEZ) and Fukushima (FEZ). Ambient dose rates were similar for both sampling gradients ranging from 0.5 to 80 μGy/h at plant height. The hypothesis was tested whether a history of several generations of plants growing in enhanced radiation exposure conditions would have led to changes in genome-wide DNA methylation. However, no differences were found in the global percentage of 5-methylated cytosines in Capsella bursa pastoris plants sampled in FEZ. On the other hand a significant decrease in whole genome methylation percentage in Arabidopsis thaliana plants was found in CEZ mainly governed by the highest exposed plants. These data support a link between exposure to changed environmental conditions and changes genome methylation. In addition to methylation the activity concentration of different radionuclides, 137Cs, 90Sr, 241Am and Pu-238,239,240 for CEZ and 137, 134Cs for FEZ, was analysed in both soil and plant samples. The ratio of 5.6 between 137Cs compared to 134Cs was as expected five years after the FEZ accident. For CEZ 137Cs is the most abundant polluting radionuclide in soil followed by 90Sr. Whereas 241Am and Pu-isotopes are only marginally present. In the plant tissue, however, higher levels of Sr than Cs were retrieved due to a high uptake of 90Sr in the plants. The 90Sr transfer factors ranged in CEZ from 5 to 20 (kg/kg) depending on the locality. Based on the activity concentrations of the different radionuclides the ERICA tool was used to estimate the total dose rates to the plants. It was found that for FEZ the doses was mainly contributable to the external Cs-isotopes and as such estimated total dose rates (0.13–38 μGy/h) were in the same range as the ambient measured dose rates. In strong contrast this was not true for CEZ where the total dose rate was mainly due to high uptake of the 90Sr leading to dose rates ranging from 1 to 370 μGy/h. Hence our data clearly indicate that not taking into account the internal contamination in CEZ will lead to considerable underestimation of the doses to the plants. Additionally they show that it is hard to compare the two nuclear accidental sites and one of the main reasons is the difference in contamination profile.

AB - The long-term radiological impact to the environment of the nuclear accidents in Chernobyl and Fukushima is still under discussion. In the course of spring of 2016 we sampled two Brassicacea plants, Arabidopsis thaliana and Capsella bursa-pastoris native to Ukraine and Japan, respectively, alongside a gradient of radiation within the exclusion and difficult to return zones of Chernobyl (CEZ) and Fukushima (FEZ). Ambient dose rates were similar for both sampling gradients ranging from 0.5 to 80 μGy/h at plant height. The hypothesis was tested whether a history of several generations of plants growing in enhanced radiation exposure conditions would have led to changes in genome-wide DNA methylation. However, no differences were found in the global percentage of 5-methylated cytosines in Capsella bursa pastoris plants sampled in FEZ. On the other hand a significant decrease in whole genome methylation percentage in Arabidopsis thaliana plants was found in CEZ mainly governed by the highest exposed plants. These data support a link between exposure to changed environmental conditions and changes genome methylation. In addition to methylation the activity concentration of different radionuclides, 137Cs, 90Sr, 241Am and Pu-238,239,240 for CEZ and 137, 134Cs for FEZ, was analysed in both soil and plant samples. The ratio of 5.6 between 137Cs compared to 134Cs was as expected five years after the FEZ accident. For CEZ 137Cs is the most abundant polluting radionuclide in soil followed by 90Sr. Whereas 241Am and Pu-isotopes are only marginally present. In the plant tissue, however, higher levels of Sr than Cs were retrieved due to a high uptake of 90Sr in the plants. The 90Sr transfer factors ranged in CEZ from 5 to 20 (kg/kg) depending on the locality. Based on the activity concentrations of the different radionuclides the ERICA tool was used to estimate the total dose rates to the plants. It was found that for FEZ the doses was mainly contributable to the external Cs-isotopes and as such estimated total dose rates (0.13–38 μGy/h) were in the same range as the ambient measured dose rates. In strong contrast this was not true for CEZ where the total dose rate was mainly due to high uptake of the 90Sr leading to dose rates ranging from 1 to 370 μGy/h. Hence our data clearly indicate that not taking into account the internal contamination in CEZ will lead to considerable underestimation of the doses to the plants. Additionally they show that it is hard to compare the two nuclear accidental sites and one of the main reasons is the difference in contamination profile.

KW - DNA hypermethylation

KW - exposure

KW - radiation

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/31331730

U2 - 10.1016/j.jenvrad.2018.07.012

DO - 10.1016/j.jenvrad.2018.07.012

M3 - Article

VL - 192

SP - 405

EP - 416

JO - Journal of environmental radioactivity

JF - Journal of environmental radioactivity

SN - 0265-931X

ER -

ID: 4632705