Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009

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Authors

  • Magdalena Calusinska
  • Christopher Hamilton
  • Pieter Monsieurs
  • Gregory Mathy
  • Natalie Leys
  • Fabrice Franck
  • Bernard Joris
  • Philippe Thonart
  • Serge Hiligsmann
  • Annick Wilmotte

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Abstract

In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase.

Details

Original languageEnglish
Pages (from-to)1-16
JournalBiotechnology for Biofuels
Volume8
Issue number27
DOIs
Publication statusPublished - 22 Feb 2015

Keywords

  • Bio-informatics, RNA-seq, biofuels

ID: 353791