Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009

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Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009. / Calusinska, Magdalena; Hamilton, Christopher; Monsieurs, Pieter; Mathy, Gregory; Leys, Natalie; Franck, Fabrice; Joris, Bernard; Thonart, Philippe; Hiligsmann, Serge; Wilmotte, Annick; Van Houdt, Rob (Peer reviewer); Moors, Hugo (Peer reviewer).

In: Biotechnology for Biofuels, Vol. 8, No. 27, 22.02.2015, p. 1-16.

Research output: Contribution to journalArticlepeer-review

Harvard

Calusinska, M, Hamilton, C, Monsieurs, P, Mathy, G, Leys, N, Franck, F, Joris, B, Thonart, P, Hiligsmann, S, Wilmotte, A, Van Houdt, R & Moors, H 2015, 'Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009', Biotechnology for Biofuels, vol. 8, no. 27, pp. 1-16. https://doi.org/10.1186/s13068-015-0203-5

APA

Calusinska, M., Hamilton, C., Monsieurs, P., Mathy, G., Leys, N., Franck, F., Joris, B., Thonart, P., Hiligsmann, S., Wilmotte, A., Van Houdt, R., & Moors, H. (2015). Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009. Biotechnology for Biofuels, 8(27), 1-16. https://doi.org/10.1186/s13068-015-0203-5

Author

Calusinska, Magdalena ; Hamilton, Christopher ; Monsieurs, Pieter ; Mathy, Gregory ; Leys, Natalie ; Franck, Fabrice ; Joris, Bernard ; Thonart, Philippe ; Hiligsmann, Serge ; Wilmotte, Annick ; Van Houdt, Rob ; Moors, Hugo. / Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009. In: Biotechnology for Biofuels. 2015 ; Vol. 8, No. 27. pp. 1-16.

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@article{04a0c205093c420ea2e887402d35e10d,
title = "Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009",
abstract = "In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase.",
keywords = "Bio-informatics, RNA-seq, biofuels",
author = "Magdalena Calusinska and Christopher Hamilton and Pieter Monsieurs and Gregory Mathy and Natalie Leys and Fabrice Franck and Bernard Joris and Philippe Thonart and Serge Hiligsmann and Annick Wilmotte and {Van Houdt}, Rob and Hugo Moors",
note = "Score = 10",
year = "2015",
month = feb,
day = "22",
doi = "10.1186/s13068-015-0203-5",
language = "English",
volume = "8",
pages = "1--16",
journal = "Biotechnology for Biofuels",
issn = "1754-6834",
publisher = "Springer",
number = "27",

}

RIS - Download

TY - JOUR

T1 - Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009

AU - Calusinska, Magdalena

AU - Hamilton, Christopher

AU - Monsieurs, Pieter

AU - Mathy, Gregory

AU - Leys, Natalie

AU - Franck, Fabrice

AU - Joris, Bernard

AU - Thonart, Philippe

AU - Hiligsmann, Serge

AU - Wilmotte, Annick

A2 - Van Houdt, Rob

A2 - Moors, Hugo

N1 - Score = 10

PY - 2015/2/22

Y1 - 2015/2/22

N2 - In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase.

AB - In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase.

KW - Bio-informatics

KW - RNA-seq

KW - biofuels

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_138533

UR - http://knowledgecentre.sckcen.be/so2/bibref/12292

U2 - 10.1186/s13068-015-0203-5

DO - 10.1186/s13068-015-0203-5

M3 - Article

VL - 8

SP - 1

EP - 16

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

SN - 1754-6834

IS - 27

ER -

ID: 353791