Genrisk-T project: response of the TPC-1 papillary thyroid carcinoma cell line to a range of low to high doses of irradiation

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Abstract

Our results indicate that TPC-1 cells do not undergo significant cell death but are more prone to cell cycle arrest in response to external X-irradiation. Only a small percentage of irradiated TPC-1 cells underwent apoptosis or necrosis in response to irradiation after 24 and 48 hours. On the other hand, the same cells showed a great decrease in the S phase of the cell cycle, which translates as a decrease in proliferation. Examination of the morphology of irradiated TPC-1 cells showed signs of apoptosis and cellular damage in some of the cells at 24 and 48 hours. In conclusion, our results indicate that irradiation of a human papillary thyroid carcinoma cell line, TPC-1, causes a decrease in cells in the S phase of the cell cycle through activation of the G1/S checkpoint probably caused by an increase in the translational activity of p53 through p21. Apoptosis and necrosis seem not to play as important a role in these cells' response to X-irradiation.

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Original languageEnglish
Pages (from-to)79-83
JournalCommunications in agricultural and applied biological sciences
Volume73
Issue number1
Publication statusPublished - 15 Sep 2008

Keywords

  • TPC-1, Papillary Thyroid Carcinoma, Apoptosis, Caspase-3, Bcl-2, p53, radiation, flow cytometry, proliferation

ID: 339107