Gold nanoparticles affect the antioxidant status in selected normal human cells.

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Gold nanoparticles affect the antioxidant status in selected normal human cells. / Daems, Noami; Van Hoecke, Karen; Cardinaels, Thomas; Baatout, Sarah; Aerts, An; Michiels, Carine; Lucas, Stéphane; Penninckx, Sébastien; Nelissen, Inge.

In: International Journal of Nanomedicine, Vol. 14, 09.07.2019, p. 4991–5015.

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Daems, N, Van Hoecke, K, Cardinaels, T, Baatout, S, Aerts, A, Michiels, C, Lucas, S, Penninckx, S & Nelissen, I 2019, 'Gold nanoparticles affect the antioxidant status in selected normal human cells.', International Journal of Nanomedicine, vol. 14, pp. 4991–5015. https://doi.org/10.2147/IJN.S203546

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Daems, Noami ; Van Hoecke, Karen ; Cardinaels, Thomas ; Baatout, Sarah ; Aerts, An ; Michiels, Carine ; Lucas, Stéphane ; Penninckx, Sébastien ; Nelissen, Inge. / Gold nanoparticles affect the antioxidant status in selected normal human cells. In: International Journal of Nanomedicine. 2019 ; Vol. 14. pp. 4991–5015.

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@article{753f3bac6fad4d6e9b6bcffc7e539a03,
title = "Gold nanoparticles affect the antioxidant status in selected normal human cells.",
abstract = "Purpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453). Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization. Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo. Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stress",
keywords = "Cytotoxicity, EGFR, Cetuximab, Oxidative stress",
author = "Noami Daems and {Van Hoecke}, Karen and Thomas Cardinaels and Sarah Baatout and An Aerts and Carine Michiels and St{\'e}phane Lucas and S{\'e}bastien Penninckx and Inge Nelissen",
note = "Score=10",
year = "2019",
month = "7",
day = "9",
doi = "10.2147/IJN.S203546",
language = "English",
volume = "14",
pages = "4991–5015",
journal = "International Journal of Nanomedicine",
issn = "1178-2013",
publisher = "Dove Medical Press",

}

RIS - Download

TY - JOUR

T1 - Gold nanoparticles affect the antioxidant status in selected normal human cells.

AU - Daems, Noami

AU - Van Hoecke, Karen

AU - Cardinaels, Thomas

AU - Baatout, Sarah

AU - Aerts, An

AU - Michiels, Carine

AU - Lucas, Stéphane

AU - Penninckx, Sébastien

AU - Nelissen, Inge

N1 - Score=10

PY - 2019/7/9

Y1 - 2019/7/9

N2 - Purpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453). Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization. Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo. Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stress

AB - Purpose: This study evaluates the cytotoxicity of AuNPs coated with polyallylamine (AuNPs-PAA) and conjugated or not to the epidermal growth factor receptor (EGFR)-targeting antibody Cetuximab (AuNPs-PAA-Ctxb) in normal human kidney (HK-2), liver (THLE-2) and microvascular endothelial (TIME) cells, and compares it with two cancer cell lines that are EGFR-overexpressing (A431) or EGFR-negative (MDA-MB-453). Results: Conjugation of Cetuximab to AuNPs-PAA increased the AuNPs-PAA-Ctxb interactions with cells, but reduced their cytotoxicity. TIME cells exhibited the strongest reduction in viability after exposure to AuNPs-PAA(±Ctxb), followed by THLE-2, MDA-MB-453, HK-2 and A431 cells. This cell type-dependent sensitivity was strongly correlated to the inhibition of thioredoxin reductase (TrxR) and glutathione reductase (GR), and to the depolarization of the mitochondrial membrane potential. Both are suggested to initiate apoptosis, which was indeed detected in a concentration- and time-dependent manner. The role of oxidative stress in AuNPs-PAA(±Ctxb)-induced cytotoxicity was demonstrated by co-incubation of the cells with N-acetyl L-cysteine (NAC), which significantly decreased apoptosis and mitochondrial membrane depolarization. Conclusion: This study helps to identify the cells and tissues that could be sensitive to AuNPs and deepens the understanding of the risks associated with the use of AuNPs in vivo. Keywords: cytotoxicity, EGFR, Cetuximab, oxidative stress

KW - Cytotoxicity

KW - EGFR

KW - Cetuximab

KW - Oxidative stress

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/34747078

U2 - 10.2147/IJN.S203546

DO - 10.2147/IJN.S203546

M3 - Article

VL - 14

SP - 4991

EP - 5015

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1178-2013

ER -

ID: 5393885