Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

Research output: Contribution to journalArticle

Authors

  • Jirka Jirka Grosse
  • Markus Wehland
  • Jessica Pietsch
  • Herbert Schulz
  • Katrin Saar
  • Norbert Hübner
  • Christoph Eilles
  • Johann Bauer
  • Khalil Abouelaradat
  • Sarah Baatout
  • Xiao Ma
  • Manfred Infanger
  • Ruth Hemmersbach
  • Daniela Grimm

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Abstract

This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

Details

Original languageEnglish
Pages (from-to)5124-5140
JournalThe FASEB Journal
Volume26
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • gene expression, simulated microgravity, cytokines, neoangiogenesis

ID: 384897