Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

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Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. / Jirka Grosse, Jirka; Wehland, Markus; Pietsch, Jessica; Schulz, Herbert; Saar, Katrin; Hübner, Norbert; Eilles, Christoph; Bauer, Johann; Abouelaradat, Khalil; Baatout, Sarah; Ma, Xiao; Infanger, Manfred; Hemmersbach, Ruth; Grimm, Daniela; Moreels, Marjan (Peer reviewer).

In: The FASEB Journal, Vol. 26, No. 12, 12.2012, p. 5124-5140.

Research output: Contribution to journalArticle

Harvard

Jirka Grosse, J, Wehland, M, Pietsch, J, Schulz, H, Saar, K, Hübner, N, Eilles, C, Bauer, J, Abouelaradat, K, Baatout, S, Ma, X, Infanger, M, Hemmersbach, R, Grimm, D & Moreels, M 2012, 'Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids', The FASEB Journal, vol. 26, no. 12, pp. 5124-5140. https://doi.org/10.1096/fj.12-215749

APA

Jirka Grosse, J., Wehland, M., Pietsch, J., Schulz, H., Saar, K., Hübner, N., ... Moreels, M. (2012). Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. The FASEB Journal, 26(12), 5124-5140. https://doi.org/10.1096/fj.12-215749

Vancouver

Jirka Grosse J, Wehland M, Pietsch J, Schulz H, Saar K, Hübner N et al. Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. The FASEB Journal. 2012 Dec;26(12):5124-5140. https://doi.org/10.1096/fj.12-215749

Author

Jirka Grosse, Jirka ; Wehland, Markus ; Pietsch, Jessica ; Schulz, Herbert ; Saar, Katrin ; Hübner, Norbert ; Eilles, Christoph ; Bauer, Johann ; Abouelaradat, Khalil ; Baatout, Sarah ; Ma, Xiao ; Infanger, Manfred ; Hemmersbach, Ruth ; Grimm, Daniela ; Moreels, Marjan. / Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. In: The FASEB Journal. 2012 ; Vol. 26, No. 12. pp. 5124-5140.

Bibtex - Download

@article{746762dbeaf54e189916ef4b50acb0b3,
title = "Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids",
abstract = "This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.",
keywords = "gene expression, simulated microgravity, cytokines, neoangiogenesis",
author = "{Jirka Grosse}, Jirka and Markus Wehland and Jessica Pietsch and Herbert Schulz and Katrin Saar and Norbert H{\"u}bner and Christoph Eilles and Johann Bauer and Khalil Abouelaradat and Sarah Baatout and Xiao Ma and Manfred Infanger and Ruth Hemmersbach and Daniela Grimm and Marjan Moreels",
note = "Score = 10",
year = "2012",
month = "12",
doi = "10.1096/fj.12-215749",
language = "English",
volume = "26",
pages = "5124--5140",
journal = "The FASEB Journal",
issn = "0892-6638",
publisher = "FASEB - Federation of American Society of Experimental Biology",
number = "12",

}

RIS - Download

TY - JOUR

T1 - Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

AU - Jirka Grosse, Jirka

AU - Wehland, Markus

AU - Pietsch, Jessica

AU - Schulz, Herbert

AU - Saar, Katrin

AU - Hübner, Norbert

AU - Eilles, Christoph

AU - Bauer, Johann

AU - Abouelaradat, Khalil

AU - Baatout, Sarah

AU - Ma, Xiao

AU - Infanger, Manfred

AU - Hemmersbach, Ruth

AU - Grimm, Daniela

A2 - Moreels, Marjan

N1 - Score = 10

PY - 2012/12

Y1 - 2012/12

N2 - This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

AB - This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

KW - gene expression

KW - simulated microgravity

KW - cytokines

KW - neoangiogenesis

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_125970

UR - http://knowledgecentre.sckcen.be/so2/bibref/9785

U2 - 10.1096/fj.12-215749

DO - 10.1096/fj.12-215749

M3 - Article

VL - 26

SP - 5124

EP - 5140

JO - The FASEB Journal

JF - The FASEB Journal

SN - 0892-6638

IS - 12

ER -

ID: 384897