Growing blood vessels in space: Preparation studies of the SPHEROIDS project using related ground-based studies

Research output: Contribution to journalArticle

Authors

  • Marcus Krüger
  • Sascha Kopp
  • Markus Wehland
  • Johann Bauer
  • Sarah Baatout
  • Marjan Moreels
  • Marcel Egli
  • Thomas J. Corydon
  • Manfred Infanger
  • Daniela Grimm

Institutes & Expert groups

  • Otto von Guericke University Magdeburg - Plastic, Aesthetic and Hand Surgery
  • Max Planck Institute of Biochemistry
  • Lucerne University of Applied Sciences and Arts - Space Biology Group
  • Aarhus University - Department of Biomedicine
  • Aarhus University Hospital - Department of Ophthalmology
  • Otto von Guericke University Magdeburg - Gravitational Biology and Translational Regenerative Medicine - Faculty of Medicine and Mechanical Engineering
  • UGent - Universiteit Gent - Department of Molecular Biotechnology

Documents & links

DOI

Abstract

Endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks under normal (1g) culture conditions. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we were able to observe scaffoldfree growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

Details

Original languageEnglish
Pages (from-to)267–272
JournalActa Astronautica
Volume159
DOIs
StatePublished - 21 Mar 2019

Keywords

  • Microgravity, Spaceflight, Random positioning machine, 3D growth, Spheroids, Tubular structures

ID: 5034825