Research output: Contribution to journal › Article › peer-review
Growing blood vessels in space: Preparation studies of the SPHEROIDS project using related ground-based studies. / Krüger, Marcus; Kopp, Sascha; Wehland, Markus; Bauer, Johann; Baatout, Sarah; Moreels, Marjan; Egli, Marcel; Corydon, Thomas J.; Infanger, Manfred; Grimm, Daniela.
In: Acta Astronautica, Vol. 159, 21.03.2019, p. 267–272.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Growing blood vessels in space: Preparation studies of the SPHEROIDS project using related ground-based studies
AU - Krüger, Marcus
AU - Kopp, Sascha
AU - Wehland, Markus
AU - Bauer, Johann
AU - Baatout, Sarah
AU - Moreels, Marjan
AU - Egli, Marcel
AU - Corydon, Thomas J.
AU - Infanger, Manfred
AU - Grimm, Daniela
N1 - Score=10
PY - 2019/3/21
Y1 - 2019/3/21
N2 - Endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks under normal (1g) culture conditions. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we were able to observe scaffoldfree growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.
AB - Endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks under normal (1g) culture conditions. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we were able to observe scaffoldfree growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.
KW - Microgravity
KW - Spaceflight
KW - Random positioning machine
KW - 3D growth
KW - Spheroids
KW - Tubular structures
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/33502472
U2 - 10.1016/j.actaastro.2019.03.074
DO - 10.1016/j.actaastro.2019.03.074
M3 - Article
VL - 159
SP - 267
EP - 272
JO - Acta Astronautica
JF - Acta Astronautica
SN - 0094-5765
ER -
ID: 5034825