Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.

Research output: Contribution to journalArticle

Bibtex - Download

@article{43d671a3eaf547a1813a4909dd53eb08,
title = "Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.",
abstract = "The soil bacterium Cupriavidus metallidurans CH34 contains a high number of heavy metal resistance genes making it an interesting model organism to study microbial responses to heavy metals. In this study the transcriptional response of strain CH34 was measured when challenged to sub-lethal concentrations of various essential or toxic metals. Based on the global transcriptional responses for each challenge and the overlap in upregulated genes between different metal responses, the sixteen metals were clustered in three groups. In addition, the transcriptional response of already known metal resistance genes was assessed, and new metal response gene clusters were identified. The majority of the studied metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex interplay at transcriptional level between the different metal responses. The pronounced redundancy of these metal resistant regions-as illustrated by the large number of paralogous genes-combined with the phylogenetic distribution of these metal response regions within either evolutionary related or other metal resistant bacteria, provides important insights on the recent evolutionary forces shaping this naturally soil-dwelling bacterium into a highly metal-resistant strain well adapted to harsh and anthropogenic environments.",
keywords = "bioinformatics, metal resistance, microarrays, cupriavidus metallidurans, regulatory networks",
author = "Pieter Monsieurs and Hugo Moors and {Van Houdt}, Rob and Paul Janssen and Ann Janssen and Ilse Coninx and Max Mergeay and Natalie Leys and Felice Mastroleo",
note = "Score = 10",
year = "2011",
month = "6",
day = "25",
doi = "10.1007/s10534-011-9473-y",
language = "English",
volume = "24",
pages = "1133--1151",
journal = "Biometals",
issn = "0966-0844",
publisher = "Springer",
number = "6",

}

RIS - Download

TY - JOUR

T1 - Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.

AU - Monsieurs, Pieter

AU - Moors, Hugo

AU - Van Houdt, Rob

AU - Janssen, Paul

AU - Janssen, Ann

AU - Coninx, Ilse

AU - Mergeay, Max

AU - Leys, Natalie

A2 - Mastroleo, Felice

N1 - Score = 10

PY - 2011/6/25

Y1 - 2011/6/25

N2 - The soil bacterium Cupriavidus metallidurans CH34 contains a high number of heavy metal resistance genes making it an interesting model organism to study microbial responses to heavy metals. In this study the transcriptional response of strain CH34 was measured when challenged to sub-lethal concentrations of various essential or toxic metals. Based on the global transcriptional responses for each challenge and the overlap in upregulated genes between different metal responses, the sixteen metals were clustered in three groups. In addition, the transcriptional response of already known metal resistance genes was assessed, and new metal response gene clusters were identified. The majority of the studied metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex interplay at transcriptional level between the different metal responses. The pronounced redundancy of these metal resistant regions-as illustrated by the large number of paralogous genes-combined with the phylogenetic distribution of these metal response regions within either evolutionary related or other metal resistant bacteria, provides important insights on the recent evolutionary forces shaping this naturally soil-dwelling bacterium into a highly metal-resistant strain well adapted to harsh and anthropogenic environments.

AB - The soil bacterium Cupriavidus metallidurans CH34 contains a high number of heavy metal resistance genes making it an interesting model organism to study microbial responses to heavy metals. In this study the transcriptional response of strain CH34 was measured when challenged to sub-lethal concentrations of various essential or toxic metals. Based on the global transcriptional responses for each challenge and the overlap in upregulated genes between different metal responses, the sixteen metals were clustered in three groups. In addition, the transcriptional response of already known metal resistance genes was assessed, and new metal response gene clusters were identified. The majority of the studied metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex interplay at transcriptional level between the different metal responses. The pronounced redundancy of these metal resistant regions-as illustrated by the large number of paralogous genes-combined with the phylogenetic distribution of these metal response regions within either evolutionary related or other metal resistant bacteria, provides important insights on the recent evolutionary forces shaping this naturally soil-dwelling bacterium into a highly metal-resistant strain well adapted to harsh and anthropogenic environments.

KW - bioinformatics

KW - metal resistance

KW - microarrays

KW - cupriavidus metallidurans

KW - regulatory networks

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_117613

UR - http://knowledgecentre.sckcen.be/so2/bibref/8586

U2 - 10.1007/s10534-011-9473-y

DO - 10.1007/s10534-011-9473-y

M3 - Article

VL - 24

SP - 1133

EP - 1151

JO - Biometals

JF - Biometals

SN - 0966-0844

IS - 6

ER -

ID: 230876