High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation

Research output: Contribution to journalArticlepeer-review

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High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation. / Dieriks, Birger; De Vos, Winnok; Meesen, Geert; Van Oostveldt, Kaat; De Meyer, Tim; Ghardi, Myriam; Baatout, Sarah; Van Oostveldt, Patrick.

In: Radiation Research, Vol. 172, No. 4, 25.05.2009, p. 423-436.

Research output: Contribution to journalArticlepeer-review

Harvard

Dieriks, B, De Vos, W, Meesen, G, Van Oostveldt, K, De Meyer, T, Ghardi, M, Baatout, S & Van Oostveldt, P 2009, 'High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation', Radiation Research, vol. 172, no. 4, pp. 423-436. https://doi.org/10.1667/RR1682.1

APA

Dieriks, B., De Vos, W., Meesen, G., Van Oostveldt, K., De Meyer, T., Ghardi, M., Baatout, S., & Van Oostveldt, P. (2009). High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation. Radiation Research, 172(4), 423-436. https://doi.org/10.1667/RR1682.1

Vancouver

Dieriks B, De Vos W, Meesen G, Van Oostveldt K, De Meyer T, Ghardi M et al. High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation. Radiation Research. 2009 May 25;172(4):423-436. https://doi.org/10.1667/RR1682.1

Author

Dieriks, Birger ; De Vos, Winnok ; Meesen, Geert ; Van Oostveldt, Kaat ; De Meyer, Tim ; Ghardi, Myriam ; Baatout, Sarah ; Van Oostveldt, Patrick. / High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation. In: Radiation Research. 2009 ; Vol. 172, No. 4. pp. 423-436.

Bibtex - Download

@article{a5f4ffb5841440cc88efd5fa2846656a,
title = "High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation",
abstract = "Space travel imposes risks to human health, in large part by the increased radiation levels compared to those on Earth. To understand the effects of space radiation on humans, it is important to determine the underlying cellular mechanisms. While general dosimetry describes average radiation levels accurately, it says little about the actual physiological impact and does not provide biological information about individual cellular events. In addition, there is no information about the nature and magnitude of a systemic response through extra- and intercellular communication. To assess the stress response in human fibroblasts that were sent into space with the Foton-M3 mission, we have developed a pluralistic setup to measure DNA damage and inflammation response by combining global and local dosimetry, image cytometry and multiplex array technology, thereby maximizing the scientific output. We were able to demonstrate a significant increase in DNA double-strand breaks, determined by a twofold increase of the c-H2AX signal at the level of the single cell and a threefold up-regulation of the soluble signal proteins CCL5, IL-6, IL-8, b-2 microglobulin and EN-RAGE, which are key players in the process of inflammation, in the growth medium.",
keywords = "High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation",
author = "Birger Dieriks and {De Vos}, Winnok and Geert Meesen and {Van Oostveldt}, Kaat and {De Meyer}, Tim and Myriam Ghardi and Sarah Baatout and {Van Oostveldt}, Patrick",
note = "Score = 10",
year = "2009",
month = may,
day = "25",
doi = "10.1667/RR1682.1",
language = "English",
volume = "172",
pages = "423--436",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "RADRES - Radiation Research Society",
number = "4",

}

RIS - Download

TY - JOUR

T1 - High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation

AU - Dieriks, Birger

AU - De Vos, Winnok

AU - Meesen, Geert

AU - Van Oostveldt, Kaat

AU - De Meyer, Tim

AU - Ghardi, Myriam

AU - Baatout, Sarah

AU - Van Oostveldt, Patrick

N1 - Score = 10

PY - 2009/5/25

Y1 - 2009/5/25

N2 - Space travel imposes risks to human health, in large part by the increased radiation levels compared to those on Earth. To understand the effects of space radiation on humans, it is important to determine the underlying cellular mechanisms. While general dosimetry describes average radiation levels accurately, it says little about the actual physiological impact and does not provide biological information about individual cellular events. In addition, there is no information about the nature and magnitude of a systemic response through extra- and intercellular communication. To assess the stress response in human fibroblasts that were sent into space with the Foton-M3 mission, we have developed a pluralistic setup to measure DNA damage and inflammation response by combining global and local dosimetry, image cytometry and multiplex array technology, thereby maximizing the scientific output. We were able to demonstrate a significant increase in DNA double-strand breaks, determined by a twofold increase of the c-H2AX signal at the level of the single cell and a threefold up-regulation of the soluble signal proteins CCL5, IL-6, IL-8, b-2 microglobulin and EN-RAGE, which are key players in the process of inflammation, in the growth medium.

AB - Space travel imposes risks to human health, in large part by the increased radiation levels compared to those on Earth. To understand the effects of space radiation on humans, it is important to determine the underlying cellular mechanisms. While general dosimetry describes average radiation levels accurately, it says little about the actual physiological impact and does not provide biological information about individual cellular events. In addition, there is no information about the nature and magnitude of a systemic response through extra- and intercellular communication. To assess the stress response in human fibroblasts that were sent into space with the Foton-M3 mission, we have developed a pluralistic setup to measure DNA damage and inflammation response by combining global and local dosimetry, image cytometry and multiplex array technology, thereby maximizing the scientific output. We were able to demonstrate a significant increase in DNA double-strand breaks, determined by a twofold increase of the c-H2AX signal at the level of the single cell and a threefold up-regulation of the soluble signal proteins CCL5, IL-6, IL-8, b-2 microglobulin and EN-RAGE, which are key players in the process of inflammation, in the growth medium.

KW - High Content Analysis of Human Fibroblast Cell Cultures after Exposure to Space Radiation

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_100280

UR - http://knowledgecentre.sckcen.be/so2/bibref/6081

U2 - 10.1667/RR1682.1

DO - 10.1667/RR1682.1

M3 - Article

VL - 172

SP - 423

EP - 436

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 4

ER -

ID: 287012